Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9212 and 9213 are separated by 568 nucleotides9213 and 9214 are separated by 774 nucleotides9214 and 9215 are separated by 265 nucleotides9215 and 9216 are separated by 477 nucleotides mRNA_844: 9212 - KOG1111 N-acetylglucosaminyltransferase complex, subunit PIG-A/SPT14, required for phosphatidylinositol biosynthesis/Sulfolipid synthase, at 2,022,494 to 2,024,852 9212 mRNA_845: 9213 - KOG3346 Phosphatidylethanolamine binding protein, at 2,025,421 to 2,026,759 9213 mRNA_846: 9214 - KOG2710 Rho GTPase-activating protein, at 2,027,534 to 2,029,466 9214 mRNA_847: 9215 - HMMPfam-Amino acid permease-PF13520, at 2,029,732 to 2,032,618 9215 mRNA_848: 9216 - K17989 SDS, SDH, CHA1 L-serine/L-threonine ammonia-lyase, at 2,033,096 to 2,034,366 9216
Group Condition mRNA_844 mRNA_845 mRNA_846 mRNA_847 mRNA_848
9212 9213 9214 9215 9216
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.5 -0.2 -0.9 -0.2 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 -0.2 -0.5 -0.4 +0.0
facs on lipid accumulation Sorted No BODIPY Gate +0.4 +0.0 -0.8 +0.0 -0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.6 -0.2 +0.8 -0.4 -0.3
auxotrophy Dropout Complete -0.6 +0.3 -0.4 +0.1 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 -0.3 +0.1 -0.2 -0.4
facs on lipid accumulation Sorted High BODIPY Gate +0.7 +0.0 -0.7 +0.3 -0.8
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 -0.1 +0.2 -0.2 -0.0
auxotrophy YNB Glucose 75mM Methionine -0.2 +0.0 -0.2 +0.1 +0.1
growth on ypd YPD -0.1 -0.2 +0.4 +0.0 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 -0.1 -0.5 -0.1 +0.8
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.0 +0.0 +0.1 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.0 -0.1 -0.0 +0.3 -0.0
ypd buoyancy 40 hrs Low Buoyancy -0.0 -0.2 +0.2 +0.2 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 +0.2 +0.0 +0.2 -0.2
growth on ricinoleic acid YNB Glucose +0.1 +0.1 +0.0 +0.1 +0.1
auxotrophy YNB Glucose +0.1 +0.2 -0.1 +0.0 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.2 -0.3 +0.2 +0.2
growth on oleic acid YNB Glucose +0.0 -0.0 -0.0 +0.1 +0.7
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 +0.1 +0.2 +0.2 +0.1
growth on oleic acid YNB Oleic Acid +0.3 +0.0 -0.0 -0.0 +0.7
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