Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9218 and 9219 are separated by 969 nucleotides9219 and 9220 are separated by 73 nucleotides9220 and 9221 are separated by 308 nucleotides9221 and 9222 are separated by 75 nucleotides mRNA_850: 9218 - SUPERFAMILY--SSF52047, at 2,036,866 to 2,038,448 9218 mRNA_851: 9219 - HMMPfam-Lipase (class 3)-PF01764,SUPERFAMILY--SSF53474, at 2,039,418 to 2,042,658 9219 mRNA_852: 9220 - K01923 purC phosphoribosylaminoimidazole-succinocarboxamide synthase, at 2,042,732 to 2,043,876 9220 mRNA_853: 9221 - K13125 NOSIP nitric oxide synthase-interacting protein, at 2,044,185 to 2,045,369 9221 mRNA_854: 9222 - K01620 ltaE threonine aldolase, at 2,045,445 to 2,046,969 9222
Group Condition mRNA_850 mRNA_851 mRNA_852 mRNA_853 mRNA_854
9218 9219 9220 9221 9222
ypd buoyancy 40 hrs No Buoyancy Separation -0.9 -0.5 N.D. +0.0 -0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 -0.2 N.D. -0.1 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.8 +0.2 N.D. +0.0 +0.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.6 +0.3 N.D. +0.3 -0.3
auxotrophy Dropout Complete -0.4 +0.4 N.D. -0.4 +0.1
auxotrophy YNB Glucose 75mM Arginine -0.3 +0.3 N.D. -0.6 +0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.3 -0.5 N.D. -0.3 +0.1
growth on oleic acid YNB Glucose +0.2 +0.0 N.D. -0.4 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 -0.4 N.D. +0.1 -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.0 N.D. -0.1 -0.2
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.1 N.D. +0.0 -0.3
ypd buoyancy 40 hrs Low Buoyancy -0.2 +0.2 N.D. +0.0 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 -0.5 N.D. +0.0 +0.2
growth on ricinoleic acid YNB Glucose -0.0 +0.2 N.D. +0.3 -0.2
auxotrophy YNB Glucose 75mM Methionine -0.1 +0.4 N.D. -0.3 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 +0.3 N.D. +0.3 +0.2
auxotrophy YNB Glucose +0.2 +0.3 N.D. -0.3 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +1.1 -0.4 N.D. -0.3 +0.1
growth on oleic acid YNB Oleic Acid +0.4 +0.1 N.D. -0.0 +0.0
growth on ypd YPD +0.3 -0.2 N.D. +0.7 +0.2
facs on lipid accumulation Sorted High BODIPY Gate +1.0 -0.1 N.D. +0.2 +0.2
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