Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9220 and 9221 are separated by 308 nucleotides9221 and 9222 are separated by 75 nucleotides9222 and 9223 are separated by 286 nucleotides9223 and 9224 are separated by 172 nucleotides mRNA_852: 9220 - K01923 purC phosphoribosylaminoimidazole-succinocarboxamide synthase, at 2,042,732 to 2,043,876 9220 mRNA_853: 9221 - K13125 NOSIP nitric oxide synthase-interacting protein, at 2,044,185 to 2,045,369 9221 mRNA_854: 9222 - K01620 ltaE threonine aldolase, at 2,045,445 to 2,046,969 9222 mRNA_855: 9223 - BLAST Phosphoribosyltransferase C-terminal [Ceraceosorus bo..., at 2,047,256 to 2,049,795 9223 mRNA_856: 9224 - KOG0828 Predicted E3 ubiquitin ligase, at 2,049,968 to 2,053,108 9224
Group Condition mRNA_852 mRNA_853 mRNA_854 mRNA_855 mRNA_856
9220 9221 9222 9223 9224
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.0 +0.0 -0.3 -1.7
auxotrophy YNB Glucose 75mM Arginine N.D. -0.6 +0.3 +0.1 -1.7
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.3 +0.1 -0.2 -1.3
auxotrophy YNB Glucose N.D. -0.3 +0.2 -0.1 -1.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.0 -0.6 -0.3 -0.4
ypd buoyancy 40 hrs High Buoyancy N.D. +0.0 -0.3 -0.2 -0.8
auxotrophy Dropout Complete N.D. -0.4 +0.1 -0.0 -0.8
auxotrophy YNB Glucose 75mM Methionine N.D. -0.3 +0.2 +0.0 -1.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.1 -0.2 -0.1 -0.5
growth on oleic acid YNB Glucose N.D. -0.4 +0.1 -0.1 -0.5
growth on ricinoleic acid YNB Glucose N.D. +0.3 -0.2 -0.1 -0.8
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.0 -0.1 -0.2 -0.5
growth on ypd YPD N.D. +0.7 +0.2 -0.0 -1.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.3 +0.1 +0.0 +0.0
growth on oleic acid YNB Oleic Acid N.D. -0.0 +0.0 -0.1 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.3 -0.3 -0.0 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.3 +0.2 -0.2 -0.4
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 -0.2 -0.0 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 -0.2 +0.0 +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.2 +0.2 -0.0 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.0 +0.2 -0.1 +0.8
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