Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9225 and 9226 are separated by 504 nucleotides9226 and 9227 are separated by 180 nucleotides9227 and 9228 are separated by 212 nucleotides9228 and 9229 are separated by 217 nucleotides mRNA_857: 9225 - K14565 NOP58 nucleolar protein 58, at 2,053,274 to 2,055,374 9225 mRNA_858: 9226 - K10599 PRPF19, PRP19 pre-mRNA-processing factor 19, at 2,055,879 to 2,057,799 9226 mRNA_859: 9227 - KOG1032 Uncharacterized conserved protein, contains GRAM domain, at 2,057,980 to 2,061,220 9227 mRNA_860: 9228 - HMMPfam-PX domain-PF00787,HMMPfam-BAR domain-PF03114,ProSiteProfiles-PX domain profile.-PS50195,SMART-PhoX homologous domain, present in p47phox and p40phox.-SM00312,SUPERFAMILY--SSF103657,SUPERFAMILY--SSF64268, at 2,061,433 to 2,063,574 9228 mRNA_861: 9229 - K03513 POLM DNA polymerase mu, at 2,063,792 to 2,065,447 9229
Group Condition mRNA_857 mRNA_858 mRNA_859 mRNA_860 mRNA_861
9225 9226 9227 9228 9229
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -3.3 +0.0 -2.1 +0.1
auxotrophy YNB Glucose 75mM Arginine N.D. -2.4 -0.1 -2.0 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -3.6 +0.3 -0.9 +0.4
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -1.9 +0.1 -1.8 +0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. -4.0 +0.2 +0.3 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -2.1 +0.0 -1.3 +0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. -2.2 +0.3 -1.0 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. -0.3 -0.1 -1.5 -0.7
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -1.5 -0.1 -0.7 +0.0
growth on oleic acid YNB Glucose N.D. -1.4 -0.2 -0.7 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.6 -0.1 -2.4 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -1.2 +0.3 -0.9 +0.1
growth on ricinoleic acid YNB Glucose N.D. +0.6 -0.1 -1.9 +0.2
auxotrophy YNB Glucose N.D. +0.6 -0.1 -1.0 -0.4
growth on oleic acid YNB Oleic Acid N.D. +0.1 -0.3 -0.5 +0.4
auxotrophy Dropout Complete N.D. +0.9 -0.3 -0.4 -0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. +1.9 -0.1 -0.0 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +1.4 +0.0 -0.2 +0.5
growth on ypd YPD N.D. +2.0 +0.0 +0.2 -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. +2.1 +0.1 -0.0 -0.0
ypd buoyancy 40 hrs High Buoyancy N.D. +3.1 +0.2 +0.6 -0.1
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