Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9226 and 9227 are separated by 180 nucleotides9227 and 9228 are separated by 212 nucleotides9228 and 9229 are separated by 217 nucleotides9229 and 9230 are separated by 285 nucleotides mRNA_858: 9226 - K10599 PRPF19, PRP19 pre-mRNA-processing factor 19, at 2,055,879 to 2,057,799 9226 mRNA_859: 9227 - KOG1032 Uncharacterized conserved protein, contains GRAM domain, at 2,057,980 to 2,061,220 9227 mRNA_860: 9228 - HMMPfam-PX domain-PF00787,HMMPfam-BAR domain-PF03114,ProSiteProfiles-PX domain profile.-PS50195,SMART-PhoX homologous domain, present in p47phox and p40phox.-SM00312,SUPERFAMILY--SSF103657,SUPERFAMILY--SSF64268, at 2,061,433 to 2,063,574 9228 mRNA_861: 9229 - K03513 POLM DNA polymerase mu, at 2,063,792 to 2,065,447 9229 mRNA_862: 9230 - KOG2867 Phosphotyrosyl phosphatase activator, at 2,065,733 to 2,067,114 9230
Group Condition mRNA_858 mRNA_859 mRNA_860 mRNA_861 mRNA_862
9226 9227 9228 9229 9230
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -3.3 +0.0 -2.1 +0.1 -0.2
auxotrophy YNB Glucose 75mM Arginine -2.4 -0.1 -2.0 -0.3 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -3.6 +0.3 -0.9 +0.4 -0.3
facs on lipid accumulation Sorted High BODIPY Gate -4.0 +0.2 +0.3 +0.1 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -2.1 +0.0 -1.3 +0.2 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid -1.9 +0.1 -1.8 +0.2 +0.1
auxotrophy YNB Glucose 75mM Methionine -0.3 -0.1 -1.5 -0.7 -0.3
facs on lipid accumulation Sorted No BODIPY Gate -2.2 +0.3 -1.0 -0.1 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.6 -0.1 -2.4 -0.1 -0.2
growth on oleic acid YNB Glucose -1.4 -0.2 -0.7 +0.2 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate -1.5 -0.1 -0.7 +0.0 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -1.2 +0.3 -0.9 +0.1 -0.3
growth on ricinoleic acid YNB Glucose +0.6 -0.1 -1.9 +0.2 -0.3
auxotrophy YNB Glucose +0.6 -0.1 -1.0 -0.4 -0.1
auxotrophy Dropout Complete +0.9 -0.3 -0.4 -0.3 -0.4
growth on oleic acid YNB Oleic Acid +0.1 -0.3 -0.5 +0.4 +0.1
growth on ypd YPD +2.0 +0.0 +0.2 -0.2 -0.3
ypd buoyancy 40 hrs Low Buoyancy +1.9 -0.1 -0.0 -0.3 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +1.4 +0.0 -0.2 +0.5 +0.4
ypd buoyancy 40 hrs Median Buoyancy +2.1 +0.1 -0.0 -0.0 +0.3
ypd buoyancy 40 hrs High Buoyancy +3.1 +0.2 +0.6 -0.1 +0.1
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