Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9244 and 9245 are separated by 804 nucleotides9245 and 9246 are separated by 357 nucleotides9246 and 9247 are separated by 1120 nucleotides9247 and 9248 are separated by 252 nucleotides mRNA_876: 9244 - K00297 metF, MTHFR methylenetetrahydrofolate reductase (NADPH), at 2,089,626 to 2,091,862 9244 mRNA_877: 9245 - K07425 CYP4A alkane 1-monooxygenase, at 2,092,667 to 2,095,053 9245 mRNA_878: 9246 - K14692 SLC30A5_7, ZNT5_7, MTP, MSC2 solute carrier family 30 (zinc transporter), member 5/7, at 2,095,411 to 2,098,626 9246 mRNA_879: 9247 - K12412 MCM1 pheromone receptor transcription factor, at 2,099,747 to 2,101,589 9247 mRNA_880: 9248 - K10632 BRAP BRCA1-associated protein, at 2,101,842 to 2,104,437 9248
Group Condition mRNA_876 mRNA_877 mRNA_878 mRNA_879 mRNA_880
9244 9245 9246 9247 9248
facs on lipid accumulation Sorted Low BODIPY Gate -3.7 +0.0 -0.2 -0.1 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy -4.0 +0.2 -0.2 -0.1 +0.5
facs on lipid accumulation Sorted High BODIPY Gate -3.4 +0.3 +0.2 -0.0 -0.5
facs on lipid accumulation Sorted No BODIPY Gate -2.6 -0.0 +0.1 +0.2 -0.8
buoyancy on lipid accumulation 40 hrs High Buoyancy -3.6 +0.5 +0.1 +0.0 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid -3.7 +0.2 +0.4 +0.2 -0.0
auxotrophy YNB Glucose 75mM Arginine -3.0 -0.1 +0.2 +0.0 +0.0
growth on oleic acid YNB Glucose -3.3 +0.1 +0.4 -0.2 +0.6
growth on ricinoleic acid YNB Glucose -3.0 +0.1 +0.2 +0.0 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -2.7 +0.2 +0.3 -0.0 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -1.8 +0.1 -0.1 +0.2 -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy -2.1 +0.2 -0.2 +0.1 -0.0
auxotrophy YNB Glucose -1.8 +0.0 +0.3 +0.1 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.8 -0.1 -0.1 +0.0 -0.4
growth on oleic acid YNB Oleic Acid -2.4 +0.2 +0.4 +0.1 +0.6
growth on ypd YPD +0.0 -0.3 -0.1 -0.2 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.3 +0.0 -0.0 +0.3 -0.5
ypd buoyancy 40 hrs Low Buoyancy +0.2 -0.2 +0.0 +0.1 -0.6
ypd buoyancy 40 hrs High Buoyancy -0.5 +0.2 +0.2 +0.3 -0.4
auxotrophy Dropout Complete +0.6 -0.1 +0.4 +0.0 -0.3
auxotrophy YNB Glucose 75mM Methionine +1.0 +0.0 +0.2 -0.2 -0.2
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