Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9279 and 9280 are separated by 349 nucleotides9280 and 9281 are separated by 12 nucleotides9281 and 9282 are separated by 415 nucleotides9282 and 9283 are separated by 306 nucleotides mRNA_911: 9279 - KOG1773 Stress responsive protein, at 2,177,969 to 2,178,702 9279 mRNA_912: 9280 - Hypothetical Protein, at 2,179,052 to 2,180,263 9280 mRNA_913: 9281 - Hypothetical Protein, at 2,180,276 to 2,181,730 9281 mRNA_914: 9282 - K10903 HUS1 HUS1 checkpoint protein, at 2,182,146 to 2,183,559 9282 mRNA_915: 9283 - K01950 E6.3.5.1, NADSYN1, QNS1, nadE NAD+ synthase (glutamine-hydrolysing), at 2,183,866 to 2,186,903 9283
Group Condition mRNA_911 mRNA_912 mRNA_913 mRNA_914 mRNA_915
9279 9280 9281 9282 9283
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 -0.1 -1.3 -2.3 -0.6
growth on ypd YPD -0.1 +0.4 -0.3 -1.1 -2.8
facs on lipid accumulation Sorted High BODIPY Gate -0.0 +0.5 -0.5 -1.1 -1.8
facs on lipid accumulation Sorted No BODIPY Gate +0.0 -0.3 +0.0 -0.9 -1.9
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.1 -1.7 +0.3 -1.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 -0.2 +0.2 -1.0 -1.1
growth on ricinoleic acid YNB Glucose -0.6 +0.2 -0.4 -1.3 -0.1
auxotrophy YNB Glucose -0.2 +0.2 -1.9 -0.9 +0.7
auxotrophy Dropout Complete +0.0 -0.2 -1.1 -1.1 +0.5
auxotrophy YNB Glucose 75mM Methionine -0.0 +0.2 -1.7 -0.2 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 +0.3 +0.2 -1.3 -1.3
growth on oleic acid YNB Glucose +0.4 +0.1 -1.0 -0.3 -0.7
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.2 -1.1 -0.8 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 +0.0 +0.5 -0.8 -0.6
ypd buoyancy 40 hrs Median Buoyancy +0.0 -0.2 -0.8 +0.3 -0.4
growth on oleic acid YNB Oleic Acid +0.5 -0.1 -0.2 +0.3 -0.8
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.6 +0.9 -0.6 -1.0 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 +0.3 +0.2 -0.9 -0.2
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.2 -0.3 +0.7 -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.3 +0.6 +0.0 -0.8
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 -0.1 -0.7 +0.6 +1.6
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