Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9299 and 9300 are separated by 508 nucleotides9300 and 9301 are separated by 1465 nucleotides9301 and 9302 are separated by 239 nucleotides9302 and 9303 are separated by 239 nucleotides mRNA_931: 9299 - K14794 RRP12 ribosomal RNA-processing protein 12, at 1,838 to 6,021 9299 mRNA_932: 9300 - K01803 TPI, tpiA triosephosphate isomerase (TIM), at 6,530 to 8,158 9300 mRNA_933: 9301 - HMMPfam-NDT80 / PhoG like DNA-binding family-PF05224,ProSiteProfiles-NDT80 DNA-binding domain profile.-PS51517,SUPERFAMILY--SSF49417, at 9,624 to 11,846 9301 mRNA_934: 9302 - K18726 FAF2, UBXD8 FAS-associated factor 2, at 12,086 to 14,306 9302 mRNA_935: 9303 - HMMPfam-Glycosyl Hydrolase Family 88-PF07470,SUPERFAMILY--SSF48208, at 14,546 to 16,225 9303
Group Condition mRNA_931 mRNA_932 mRNA_933 mRNA_934 mRNA_935
9299 9300 9301 9302 9303
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.4 -0.2 -0.3
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.1 -0.3 -0.2
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.2 -0.4 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.8 +0.2 +0.1
auxotrophy Dropout Complete N.D. N.D. +0.0 -0.4 -0.1
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.3 -0.2 +0.1
auxotrophy YNB Glucose N.D. N.D. -0.3 -0.1 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.5 -0.9 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.1 -0.4 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.2 +0.2 -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.3 +0.2 -0.0
growth on ypd YPD N.D. N.D. +0.8 -1.1 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.0 -0.1 +0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.1 +0.4 -0.1
growth on oleic acid YNB Glucose N.D. N.D. +0.0 +0.2 +0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.4 +0.1 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.5 -0.3 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.3 -0.1 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.6 -0.2 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.6 -0.0 +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.2 +1.0 -0.1
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