Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9351 and 9352 are separated by 575 nucleotides9352 and 9353 are separated by 177 nucleotides9353 and 9354 are separated by 749 nucleotides9354 and 9355 are separated by 534 nucleotides mRNA_983: 9351 - K02974 RP-S24e, RPS24 small subunit ribosomal protein S24e, at 130,660 to 132,036 9351 mRNA_984: 9352 - Hypothetical Protein, at 132,612 to 133,421 9352 mRNA_985: 9353 - K14399 CLP1, HERB polyribonucleotide 5'-hydroxyl-kinase, at 133,599 to 135,746 9353 mRNA_986: 9354 - K03781 katE, CAT, catB, srpA catalase, at 136,496 to 139,711 9354 mRNA_987: 9355 - K04773 sppA protease IV, at 140,246 to 143,358 9355
Group Condition mRNA_983 mRNA_984 mRNA_985 mRNA_986 mRNA_987
9351 9352 9353 9354 9355
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.4 N.D. -0.2 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.4 N.D. +0.2 -0.4
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 N.D. -0.1 -0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.2 N.D. -0.4 +0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.0 N.D. -0.2 -0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 N.D. -0.0 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.0 N.D. -0.0 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.4 N.D. -0.0 +0.4
growth on ypd YPD N.D. -0.0 N.D. +0.2 -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.2 N.D. -0.0 +0.0
auxotrophy Dropout Complete N.D. +0.3 N.D. -0.1 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.0 N.D. +0.2 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.1 N.D. +0.3 +0.1
growth on ricinoleic acid YNB Glucose N.D. +0.2 N.D. -0.0 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.2 N.D. +0.1 +0.0
growth on oleic acid YNB Oleic Acid N.D. +0.3 N.D. +0.2 -0.0
auxotrophy YNB Glucose 75mM Methionine N.D. +0.6 N.D. -0.0 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.2 N.D. +0.2 +0.2
auxotrophy YNB Glucose N.D. +0.5 N.D. +0.2 -0.0
growth on oleic acid YNB Glucose N.D. +0.4 N.D. +0.1 +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. +0.6 N.D. +0.1 +0.0
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