Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9359 and 9360 are separated by 378 nucleotides9360 and 9361 are separated by 1365 nucleotides9361 and 9362 are separated by 704 nucleotides9362 and 9363 are separated by 676 nucleotides mRNA_991: 9359 - HMMPfam-Uncharacterised protein family, YAP/Alf4/glomulin-PF08568, at 147,829 to 149,137 9359 mRNA_992: 9360 - HMMPfam-OPT oligopeptide transporter protein-PF03169, at 149,516 to 150,268 9360 mRNA_993: 9361 - HMMPfam-Zinc finger, C2H2 type-PF00096,HMMPfam-C2H2-type zinc finger-PF13894,ProSitePatterns-Zinc finger C2H2 type domain signature.-PS00028,ProSiteProfiles-Zinc finger C2H2 type domain profile.-PS50157,SMART-zinc finger-SM00355,SUPERFAMILY--SSF57667, at 151,634 to 153,360 9361 mRNA_994: 9362 - K08513 VAMP4 vesicle-associated membrane protein 4, at 154,065 to 154,836 9362 mRNA_995: 9363 - KOG0065 Pleiotropic drug resistance proteins (PDR1-15), ABC superfamily, at 155,513 to 160,267 9363
Group Condition mRNA_991 mRNA_992 mRNA_993 mRNA_994 mRNA_995
9359 9360 9361 9362 9363
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.1 -0.5 -1.2 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.6 +0.0 +0.1 -0.3
growth on oleic acid YNB Oleic Acid N.D. +0.2 -0.3 -0.3 -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. +0.0 -0.1 -0.4 -0.1
growth on oleic acid YNB Glucose N.D. +0.1 -0.2 -0.0 -0.4
auxotrophy YNB Glucose 75mM Arginine N.D. +0.2 -0.1 -0.4 +0.0
ypd buoyancy 40 hrs High Buoyancy N.D. -0.3 +0.2 -0.1 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.3 -0.3 -0.4 +0.0
growth on ricinoleic acid YNB Glucose N.D. +0.4 -0.5 -0.1 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.0 +0.0 +0.4 -0.4
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.2 -0.1 +0.3 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.2 +0.2 -0.0 -0.4
auxotrophy Dropout Complete N.D. +0.4 -0.1 -0.4 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.7 +0.1 +0.6 +0.0
auxotrophy YNB Glucose N.D. +0.4 -0.1 -0.5 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.1 +0.1 -0.6 +0.4
growth on ypd YPD N.D. -0.3 +0.1 +0.1 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.1 +0.1 +0.3 -0.4
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.2 +0.4 -0.3 +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.2 -0.2 +0.3 -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.2 +0.4 -0.2 +0.3
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