Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9368 and 9369 are separated by 173 nucleotides9369 and 9370 are separated by 562 nucleotides9370 and 9371 are separated by 1102 nucleotides9371 and 9372 are separated by 1022 nucleotides mRNA_1000: 9368 - BLAST glycosyltransferase 2 [Moniliophthora roreri MCA ..., at 171,507 to 175,243 9368 mRNA_1001: 9369 - BLAST transmembrane protein, putative [Rhizoctonia solani A..., at 175,417 to 177,383 9369 mRNA_1002: 9370 - KOG3435 Mitochondrial/chloroplast ribosomal protein L54/L37, at 177,946 to 178,405 9370 mRNA_1003: 9371 - HMMPfam-Protein of unknown function (DUF2841)-PF11001, at 179,508 to 182,769 9371 mRNA_1004: 9372 - K02220 CLB1_2 G2/mitotic-specific cyclin 1/2, at 183,792 to 186,232 9372
Group Condition mRNA_1000 mRNA_1001 mRNA_1002 mRNA_1003 mRNA_1004
9368 9369 9370 9371 9372
ypd buoyancy 40 hrs No Buoyancy Separation -1.4 +0.0 N.D. -0.6 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.7 -0.3 N.D. +0.3 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.4 N.D. -0.0 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.4 -0.2 N.D. -0.2 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.0 N.D. -0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.0 +0.1 N.D. +0.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.2 -0.0 N.D. -0.0 N.D.
growth on ypd YPD +0.4 -0.1 N.D. +0.0 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.5 -0.1 N.D. +0.0 N.D.
auxotrophy YNB Glucose +0.4 +0.0 N.D. +0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.3 +0.1 N.D. +0.3 N.D.
growth on ricinoleic acid YNB Glucose +0.3 +0.3 N.D. +0.4 N.D.
growth on oleic acid YNB Glucose +0.6 +0.1 N.D. +0.3 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.8 +0.1 N.D. +0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.5 +0.1 N.D. +0.4 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +1.0 -0.1 N.D. +0.2 N.D.
auxotrophy Dropout Complete +1.0 +0.1 N.D. +0.3 N.D.
ypd buoyancy 40 hrs Low Buoyancy +1.4 -0.2 N.D. +0.1 N.D.
growth on oleic acid YNB Oleic Acid +1.3 -0.0 N.D. +0.4 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +1.9 +0.2 N.D. +0.7 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +2.1 +0.1 N.D. +1.0 N.D.
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