Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9373 and 9374 are separated by 240 nucleotides9374 and 9375 are separated by 354 nucleotides9375 and 9376 are separated by 341 nucleotides9376 and 9377 are separated by 285 nucleotides mRNA_1005: 9373 - K08794 CAMK1 calcium/calmodulin-dependent protein kinase I, at 186,567 to 188,548 9373 mRNA_1006: 9374 - KOG4569 Predicted lipase, at 188,789 to 191,273 9374 mRNA_1007: 9375 - HMMPfam-S1/P1 Nuclease-PF02265,SUPERFAMILY--SSF48537, at 191,628 to 193,033 9375 mRNA_1008: 9376 - BLAST expressed protein [Cryptococcus neoformans var. neofo..., at 193,375 to 193,873 9376 mRNA_1009: 9377 - K12659 ARG56 N-acetyl-gamma-glutamyl-phosphate reductase / acetylglutamate kinase, at 194,159 to 197,453 9377
Group Condition mRNA_1005 mRNA_1006 mRNA_1007 mRNA_1008 mRNA_1009
9373 9374 9375 9376 9377
auxotrophy YNB Glucose 75mM Methionine +0.3 +0.2 -0.4 -0.4 -4.7
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 +0.1 -0.5 -0.3 -3.9
facs on lipid accumulation Sorted Low BODIPY Gate -0.6 +0.2 -0.9 -0.2 -3.2
growth on oleic acid YNB Glucose +0.0 +0.2 +0.1 -0.2 -4.8
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 +0.1 -0.2 -0.2 -4.0
growth on ricinoleic acid YNB Glucose -0.2 +0.3 -0.3 -0.2 -4.0
facs on lipid accumulation Sorted High BODIPY Gate +0.1 +0.2 -0.8 -0.0 -3.5
auxotrophy YNB Glucose +0.4 +0.1 +0.6 -0.2 -4.4
facs on lipid accumulation Sorted No BODIPY Gate -0.1 +0.2 +0.2 -0.3 -3.2
growth on oleic acid YNB Oleic Acid -0.0 -0.0 +0.4 -0.1 -3.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 +0.1 +1.7 +0.0 -4.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 +0.0 +1.0 -0.2 -2.9
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 +0.0 +1.6 +0.4 -4.0
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 -0.3 -1.0 +0.1 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 +0.1 +2.1 -0.4 -2.8
ypd buoyancy 40 hrs Low Buoyancy -0.3 -0.0 -0.6 +0.7 -0.3
ypd buoyancy 40 hrs High Buoyancy -0.3 -0.1 +0.1 +0.1 -0.3
growth on ypd YPD +0.2 +0.1 -0.4 -0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.0 -0.0 +0.2 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.3 +0.2 -0.1 +0.1 -0.1
auxotrophy Dropout Complete +0.3 +0.2 +0.1 +0.0 -0.1
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