Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9386 and 9387 are separated by 364 nucleotides9387 and 9388 are separated by 237 nucleotides9388 and 9389 are separated by 453 nucleotides9389 and 9390 are separated by 492 nucleotides mRNA_1018: 9386 - KOG3488 Dolichol phosphate-mannose regulatory protein (DPM2), at 219,335 to 219,910 9386 mRNA_1019: 9387 - K02835 prfA, MTRF1, MRF1 peptide chain release factor 1, at 220,275 to 221,904 9387 mRNA_1020: 9388 - K01527 EGD1, BTF3 nascent polypeptide-associated complex subunit beta, at 222,142 to 223,175 9388 mRNA_1021: 9389 - KOG3457 Sec61 protein translocation complex, beta subunit, at 223,629 to 224,377 9389 mRNA_1022: 9390 - KOG1550 Extracellular protein SEL-1 and related proteins, at 224,870 to 227,887 9390
Group Condition mRNA_1018 mRNA_1019 mRNA_1020 mRNA_1021 mRNA_1022
9386 9387 9388 9389 9390
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.6 N.D. -1.0
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.2 N.D. -1.1
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.4 N.D. -0.9
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.4 N.D. -0.8
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.3 N.D. -0.8
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.7 N.D. -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.2 N.D. -0.8
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.1 N.D. -0.6
auxotrophy Dropout Complete N.D. N.D. -0.1 N.D. -0.5
growth on oleic acid YNB Glucose N.D. N.D. -0.4 N.D. -0.2
auxotrophy YNB Glucose N.D. N.D. +0.1 N.D. -0.7
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.0 N.D. -0.4
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.1 N.D. -0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.1 N.D. -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.3 N.D. -0.4
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.0 N.D. -0.1
growth on ypd YPD N.D. N.D. -0.1 N.D. +0.0
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.5 N.D. -0.4
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.0 N.D. +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.6 N.D. -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.5 N.D. -0.1
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