Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9404 and 9405 are separated by 113 nucleotides9405 and 9406 are separated by 583 nucleotides9406 and 9407 are separated by 115 nucleotides9407 and 9408 are separated by 160 nucleotides mRNA_1036: 9404 - K12590 RRP46, EXOSC5 exosome complex component RRP46, at 253,170 to 254,250 9404 mRNA_1037: 9405 - K12160 SUMO, SMT3 small ubiquitin-related modifier, at 254,364 to 255,172 9405 mRNA_1038: 9406 - HMMPfam-Protein of unknown function (DUF2034)-PF10356, at 255,756 to 256,586 9406 mRNA_1039: 9407 - K18723 GLE1 nucleoporin GLE1, at 256,702 to 258,919 9407 mRNA_1040: 9408 - HMMPfam-NADH(P)-binding-PF13460,SUPERFAMILY--SSF51735, at 259,080 to 260,427 9408
Group Condition mRNA_1036 mRNA_1037 mRNA_1038 mRNA_1039 mRNA_1040
9404 9405 9406 9407 9408
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -1.8 N.D. -0.2
auxotrophy Dropout Complete N.D. N.D. -1.0 N.D. -0.3
auxotrophy YNB Glucose N.D. N.D. -0.7 N.D. -0.1
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.5 N.D. -0.2
growth on oleic acid YNB Glucose N.D. N.D. -0.3 N.D. -0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.3 N.D. -0.3
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.2 N.D. -0.5
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.3 N.D. -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.1 N.D. -0.2
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.1 N.D. -0.2
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.2 N.D. -0.3
growth on ypd YPD N.D. N.D. +0.1 N.D. -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.0 N.D. -0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.0 N.D. +0.0
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.1 N.D. -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.2 N.D. -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.2 N.D. +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.2 N.D. +0.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.3 N.D. -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.6 N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.6 N.D. +0.2
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