Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9412 and 9413 are separated by 539 nucleotides9413 and 9414 are separated by 1462 nucleotides9414 and 9415 are separated by 413 nucleotides9415 and 9416 are separated by 184 nucleotides mRNA_1044: 9412 - K07765 MBTPS2 S2P endopeptidase, at 266,610 to 267,641 9412 mRNA_1045: 9413 - Hypothetical Protein, at 268,181 to 268,879 9413 mRNA_1046: 9414 - K11346 ING4 inhibitor of growth protein 4, at 270,342 to 271,774 9414 mRNA_1047: 9415 - K19054 FXN frataxin, at 272,188 to 272,699 9415 mRNA_1048: 9416 - KOG1812 Predicted E3 ubiquitin ligase, at 272,884 to 273,999 9416
Group Condition mRNA_1044 mRNA_1045 mRNA_1046 mRNA_1047 mRNA_1048
9412 9413 9414 9415 9416
facs on lipid accumulation Sorted High BODIPY Gate -0.6 N.D. +0.0 N.D. -0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 N.D. -0.2 N.D. +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 N.D. +0.0 N.D. -0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 N.D. -0.0 N.D. +0.0
growth on ricinoleic acid YNB Glucose +0.2 N.D. -0.3 N.D. -0.0
growth on oleic acid YNB Oleic Acid -0.3 N.D. +0.1 N.D. +0.0
auxotrophy YNB Glucose 75mM Methionine +0.4 N.D. -0.3 N.D. -0.2
growth on ypd YPD -0.0 N.D. -0.0 N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine +0.4 N.D. -0.2 N.D. -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.3 N.D. -0.3 N.D. +0.1
growth on oleic acid YNB Glucose +0.2 N.D. -0.1 N.D. +0.0
auxotrophy Dropout Complete +0.5 N.D. -0.2 N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy -0.0 N.D. +0.1 N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.3 N.D. +0.3 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 N.D. -0.5 N.D. +0.7
auxotrophy YNB Glucose +0.7 N.D. -0.3 N.D. +0.0
ypd buoyancy 40 hrs Median Buoyancy +0.4 N.D. -0.0 N.D. +0.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 N.D. +0.8 N.D. +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 N.D. +0.6 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 N.D. +0.8 N.D. +0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.7 N.D. +0.6 N.D. +0.5
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