Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9414 and 9415 are separated by 413 nucleotides9415 and 9416 are separated by 184 nucleotides9416 and 9417 are separated by 76 nucleotides9417 and 9418 are separated by 278 nucleotides mRNA_1046: 9414 - K11346 ING4 inhibitor of growth protein 4, at 270,342 to 271,774 9414 mRNA_1047: 9415 - K19054 FXN frataxin, at 272,188 to 272,699 9415 mRNA_1048: 9416 - KOG1812 Predicted E3 ubiquitin ligase, at 272,884 to 273,999 9416 mRNA_1049: 9417 - K12878 THOC1 THO complex subunit 1, at 274,076 to 276,723 9417 mRNA_1050: 9418 - Hypothetical Protein, at 277,002 to 277,499 9418
Group Condition mRNA_1046 mRNA_1047 mRNA_1048 mRNA_1049 mRNA_1050
9414 9415 9416 9417 9418
growth on oleic acid YNB Oleic Acid +0.1 N.D. +0.0 -0.4 -0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.0 N.D. -0.2 +0.1 -0.3
auxotrophy Dropout Complete -0.2 N.D. -0.1 +0.3 -0.2
growth on ypd YPD -0.0 N.D. -0.1 +0.0 -0.2
auxotrophy YNB Glucose 75mM Methionine -0.3 N.D. -0.2 +0.2 +0.2
ypd buoyancy 40 hrs Low Buoyancy -0.3 N.D. +0.1 +0.1 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.2 N.D. -0.2 +0.4 -0.2
growth on ricinoleic acid YNB Glucose -0.3 N.D. -0.0 -0.1 +0.4
auxotrophy YNB Glucose -0.3 N.D. +0.0 +0.3 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.6 N.D. -0.2 +0.2 -0.6
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 N.D. -0.3 +0.1 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 N.D. +0.2 -0.3 +0.3
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. +0.1 +0.2 -0.3
ypd buoyancy 40 hrs Median Buoyancy -0.0 N.D. +0.3 -0.1 +0.1
growth on oleic acid YNB Glucose -0.1 N.D. +0.0 +0.0 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 N.D. +0.0 -0.0 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.5 N.D. +0.7 +0.5 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.8 N.D. +0.5 +0.2 -0.4
facs on lipid accumulation Sorted No BODIPY Gate +0.3 N.D. +0.2 +0.1 +0.5
facs on lipid accumulation Sorted Low BODIPY Gate +0.8 N.D. +0.2 +0.0 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.6 N.D. +0.5 +0.4 -0.1
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