Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9419 and 9420 are separated by 364 nucleotides9420 and 9421 are separated by 244 nucleotides9421 and 9422 are separated by 112 nucleotides9422 and 9423 are separated by 1268 nucleotides mRNA_1051: 9419 - SUPERFAMILY--SSF54928, at 277,584 to 281,665 9419 mRNA_1052: 9420 - K10896 FANCM fanconi anemia group M protein, at 282,030 to 286,830 9420 mRNA_1053: 9421 - KOG3856 Uncharacterized conserved protein, at 287,075 to 287,902 9421 mRNA_1054: 9422 - K09419 HSFF heat shock transcription factor, other eukaryote, at 288,015 to 290,756 9422 mRNA_1055: 9423 - KOG0590 Checkpoint kinase and related serine/threonine protein kinases, at 292,025 to 293,259 9423
Group Condition mRNA_1051 mRNA_1052 mRNA_1053 mRNA_1054 mRNA_1055
9419 9420 9421 9422 9423
growth on ypd YPD -0.3 -0.3 N.D. N.D. -0.9
growth on oleic acid YNB Oleic Acid -0.1 -0.2 N.D. N.D. -0.9
growth on oleic acid YNB Glucose -0.2 -0.2 N.D. N.D. -0.4
growth on ricinoleic acid YNB Glucose -0.1 +0.1 N.D. N.D. -0.8
facs on lipid accumulation Sorted High BODIPY Gate -0.1 +0.1 N.D. N.D. -0.5
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.0 N.D. N.D. -0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 +0.4 N.D. N.D. -0.9
auxotrophy YNB Glucose -0.1 -0.1 N.D. N.D. -0.2
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.3 N.D. N.D. -0.6
auxotrophy YNB Glucose 75mM Methionine -0.2 -0.2 N.D. N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.0 +0.2 N.D. N.D. -0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 -0.1 N.D. N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine -0.0 -0.0 N.D. N.D. -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.0 N.D. N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 +0.1 N.D. N.D. -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 -0.1 N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.2 N.D. N.D. +0.2
ypd buoyancy 40 hrs Low Buoyancy +0.2 +0.3 N.D. N.D. -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +0.2 N.D. N.D. +0.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -0.1 N.D. N.D. +0.9
auxotrophy Dropout Complete -0.1 +0.0 N.D. N.D. +0.8
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