Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9421 and 9422 are separated by 112 nucleotides9422 and 9423 are separated by 1268 nucleotides9423 and 9424 are separated by 1271 nucleotides9424 and 9425 are separated by 40 nucleotides mRNA_1053: 9421 - KOG3856 Uncharacterized conserved protein, at 287,075 to 287,902 9421 mRNA_1054: 9422 - K09419 HSFF heat shock transcription factor, other eukaryote, at 288,015 to 290,756 9422 mRNA_1055: 9423 - KOG0590 Checkpoint kinase and related serine/threonine protein kinases, at 292,025 to 293,259 9423 mRNA_1056: 9424 - Hypothetical Protein, at 294,531 to 295,734 9424 mRNA_1057: 9425 - K00654 SPT serine palmitoyltransferase, at 295,775 to 298,601 9425
Group Condition mRNA_1053 mRNA_1054 mRNA_1055 mRNA_1056 mRNA_1057
9421 9422 9423 9424 9425
growth on ypd YPD N.D. N.D. -0.9 -0.9 N.D.
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.8 -0.4 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.5 -0.4 N.D.
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.9 +0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.9 +0.1 N.D.
auxotrophy YNB Glucose N.D. N.D. -0.2 -0.3 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.1 -0.4 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.6 +0.3 N.D.
growth on oleic acid YNB Glucose N.D. N.D. -0.4 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.1 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.1 -0.3 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.3 +0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.0 -0.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.2 -0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.2 +0.3 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.0 +0.3 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.1 +0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.4 +0.7 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.3 +0.5 N.D.
auxotrophy Dropout Complete N.D. N.D. +0.8 +0.4 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.9 +0.5 N.D.
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