Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9423 and 9424 are separated by 1271 nucleotides9424 and 9425 are separated by 40 nucleotides9425 and 9426 are separated by 321 nucleotides9426 and 9427 are separated by 780 nucleotides mRNA_1055: 9423 - KOG0590 Checkpoint kinase and related serine/threonine protein kinases, at 292,025 to 293,259 9423 mRNA_1056: 9424 - Hypothetical Protein, at 294,531 to 295,734 9424 mRNA_1057: 9425 - K00654 SPT serine palmitoyltransferase, at 295,775 to 298,601 9425 mRNA_1058: 9426 - HMMPfam-Protein of unknown function (DUF1115)-PF06544,PIRSF--PIRSF038021,SUPERFAMILY--SSF54495, at 298,923 to 300,090 9426 mRNA_1059: 9427 - BLAST expressed protein [Cryptococcus neoformans var. neofo..., at 300,871 to 301,562 9427
Group Condition mRNA_1055 mRNA_1056 mRNA_1057 mRNA_1058 mRNA_1059
9423 9424 9425 9426 9427
growth on ypd YPD -0.9 -0.9 N.D. -0.4 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -0.4 N.D. -0.5 -0.8
growth on oleic acid YNB Oleic Acid -0.9 +0.1 N.D. -0.3 -0.5
growth on ricinoleic acid YNB Glucose -0.8 -0.4 N.D. +0.2 -0.4
facs on lipid accumulation Sorted High BODIPY Gate -0.5 -0.4 N.D. -0.1 -0.1
auxotrophy YNB Glucose -0.2 -0.3 N.D. +0.1 -0.4
growth on oleic acid YNB Glucose -0.4 +0.1 N.D. +0.1 -0.5
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.1 N.D. -0.3 -0.6
ypd buoyancy 40 hrs High Buoyancy -0.6 +0.3 N.D. +0.3 -0.3
facs on lipid accumulation Sorted No BODIPY Gate -0.3 +0.1 N.D. -0.0 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.0 +0.3 N.D. -0.1 -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 -0.1 N.D. +0.1 +0.0
ypd buoyancy 40 hrs Low Buoyancy -0.0 -0.0 N.D. +0.3 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.9 +0.1 N.D. +0.8 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 +0.3 N.D. +0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.4 +0.7 N.D. +0.3 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.2 N.D. +0.4 +0.0
auxotrophy Dropout Complete +0.8 +0.4 N.D. +0.1 -0.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -0.3 N.D. +0.6 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.9 +0.5 N.D. -0.4 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 +0.5 N.D. +0.4 -0.1
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