Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9424 and 9425 are separated by 40 nucleotides9425 and 9426 are separated by 321 nucleotides9426 and 9427 are separated by 780 nucleotides9427 and 9428 are separated by 243 nucleotides mRNA_1056: 9424 - Hypothetical Protein, at 294,531 to 295,734 9424 mRNA_1057: 9425 - K00654 SPT serine palmitoyltransferase, at 295,775 to 298,601 9425 mRNA_1058: 9426 - HMMPfam-Protein of unknown function (DUF1115)-PF06544,PIRSF--PIRSF038021,SUPERFAMILY--SSF54495, at 298,923 to 300,090 9426 mRNA_1059: 9427 - BLAST expressed protein [Cryptococcus neoformans var. neofo..., at 300,871 to 301,562 9427 mRNA_1060: 9428 - K01530 E3.6.3.1 phospholipid-translocating ATPase, at 301,806 to 307,768 9428
Group Condition mRNA_1056 mRNA_1057 mRNA_1058 mRNA_1059 mRNA_1060
9424 9425 9426 9427 9428
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 N.D. -0.5 -0.8 +0.4
growth on ypd YPD -0.9 N.D. -0.4 +0.1 +0.1
growth on oleic acid YNB Oleic Acid +0.1 N.D. -0.3 -0.5 +0.1
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. -0.3 -0.6 +0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.4 N.D. -0.1 -0.1 +0.0
growth on ricinoleic acid YNB Glucose -0.4 N.D. +0.2 -0.4 +0.2
growth on oleic acid YNB Glucose +0.1 N.D. +0.1 -0.5 -0.0
auxotrophy YNB Glucose -0.3 N.D. +0.1 -0.4 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. +0.1 +0.0 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.5 N.D. -0.4 -0.1 -0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. -0.0 -0.1 +0.0
auxotrophy YNB Glucose 75mM Arginine +0.3 N.D. -0.1 -0.5 +0.3
auxotrophy Dropout Complete +0.4 N.D. +0.1 -0.7 +0.3
ypd buoyancy 40 hrs Low Buoyancy -0.0 N.D. +0.3 -0.3 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.3 N.D. +0.3 -0.3 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 N.D. +0.4 +0.0 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 N.D. +0.8 +0.0 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 N.D. +0.1 -0.1 +0.3
ypd buoyancy 40 hrs Median Buoyancy +0.7 N.D. +0.3 -0.3 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.5 N.D. +0.4 -0.1 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 N.D. +0.6 +0.3 +0.0
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