Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9425 and 9426 are separated by 321 nucleotides9426 and 9427 are separated by 780 nucleotides9427 and 9428 are separated by 243 nucleotides9428 and 9429 are separated by 734 nucleotides mRNA_1057: 9425 - K00654 SPT serine palmitoyltransferase, at 295,775 to 298,601 9425 mRNA_1058: 9426 - HMMPfam-Protein of unknown function (DUF1115)-PF06544,PIRSF--PIRSF038021,SUPERFAMILY--SSF54495, at 298,923 to 300,090 9426 mRNA_1059: 9427 - BLAST expressed protein [Cryptococcus neoformans var. neofo..., at 300,871 to 301,562 9427 mRNA_1060: 9428 - K01530 E3.6.3.1 phospholipid-translocating ATPase, at 301,806 to 307,768 9428 mRNA_1061: 9429 - BLAST heat shock 70 kDa protein 12A [Rhizoctonia solani AG-..., at 308,503 to 311,284 9429
Group Condition mRNA_1057 mRNA_1058 mRNA_1059 mRNA_1060 mRNA_1061
9425 9426 9427 9428 9429
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.5 -0.8 +0.4 -0.3
growth on oleic acid YNB Glucose N.D. +0.1 -0.5 -0.0 -0.5
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.4 -0.1 -0.2 -0.2
growth on oleic acid YNB Oleic Acid N.D. -0.3 -0.5 +0.1 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.4 -0.1 -0.1 -0.8
auxotrophy YNB Glucose 75mM Methionine N.D. -0.3 -0.6 +0.3 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.8 +0.0 -0.4 -1.0
ypd buoyancy 40 hrs High Buoyancy N.D. +0.3 -0.3 -0.0 -0.5
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.0 -0.1 +0.0 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.1 +0.0 -0.2 -0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.1 -0.1 +0.0 -0.3
growth on ypd YPD N.D. -0.4 +0.1 +0.1 -0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.3 -0.3 -0.0 -0.1
auxotrophy Dropout Complete N.D. +0.1 -0.7 +0.3 +0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.3 -0.3 +0.1 -0.2
auxotrophy YNB Glucose 75mM Arginine N.D. -0.1 -0.5 +0.3 +0.2
growth on ricinoleic acid YNB Glucose N.D. +0.2 -0.4 +0.2 +0.0
auxotrophy YNB Glucose N.D. +0.1 -0.4 +0.4 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.4 +0.0 +0.0 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.1 -0.1 +0.3 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.6 +0.3 +0.0 -0.4
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