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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
9429 and 9430 are separated by 94 nucleotides
9430 and 9431 are separated by 622 nucleotides
9431 and 9432 are separated by 412 nucleotides
9432 and 9433 are separated by 381 nucleotides
mRNA_1061: 9429 - BLAST heat shock 70 kDa protein 12A [Rhizoctonia solani AG-..., at 308,503 to 311,284
9429
mRNA_1062: 9430 - K10742 DNA2 DNA replication ATP-dependent helicase Dna2, at 311,379 to 314,968
9430
mRNA_1063: 9431 - BLAST DNA replication ATP-dependent helicase Dna2 [Rhod..., at 315,591 to 316,093
9431
mRNA_1064: 9432 - K05533 ANP1 mannan polymerase II complex ANP1 subunit, at 316,506 to 317,869
9432
mRNA_1065: 9433 - Hypothetical Protein, at 318,251 to 319,455
9433
Group
Condition
mRNA
_1061
mRNA
_1062
mRNA
_1063
mRNA
_1064
mRNA
_1065
9429
9430
9431
9432
9433
ypd buoyancy
40 hrs No Buoyancy Separation
-1.0
-0.5
-0.6
-0.7
+0.4
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.8
-0.3
+0.1
+0.0
-1.0
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.3
-0.2
+0.2
-0.6
-0.5
facs on lipid accumulation
Sorted No BODIPY Gate
-0.3
-0.2
-0.5
+0.0
-0.4
auxotrophy
Dropout Complete
+0.2
-0.2
-0.7
+0.1
-0.3
auxotrophy
YNB Glucose 75mM Methionine
-0.0
-0.1
-0.8
+0.1
-0.1
auxotrophy
YNB Glucose
+0.1
-0.1
-0.6
+0.1
-0.5
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.4
-0.2
+0.1
-0.1
-0.4
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.3
-0.2
+0.1
+0.2
-0.6
growth on oleic acid
YNB Glucose
-0.5
+0.1
-0.1
+0.2
-0.3
growth on oleic acid
YNB Oleic Acid
-0.2
-0.1
-0.6
+0.3
-0.1
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.2
-0.2
-0.9
+0.4
+0.3
growth on ricinoleic acid
YNB Glucose
+0.0
-0.1
-0.7
+0.2
+0.2
ypd buoyancy
40 hrs High Buoyancy
-0.5
-0.1
+0.3
+0.0
-0.0
auxotrophy
YNB Glucose 75mM Arginine
+0.2
-0.0
-0.5
+0.3
-0.2
growth on ypd
YPD
-0.0
-0.3
+0.3
-0.0
-0.0
facs on lipid accumulation
Sorted High BODIPY Gate
-0.3
+0.2
-0.4
+0.4
+0.1
ypd buoyancy
40 hrs Low Buoyancy
-0.2
-0.1
-0.2
+0.3
+0.2
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.1
-0.0
-0.9
+1.0
+0.2
ypd buoyancy
40 hrs Median Buoyancy
-0.1
-0.0
+0.1
-0.0
+0.3
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.3
-0.1
-1.0
+1.0
+0.8
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