Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9441 and 9442 are separated by 765 nucleotides9442 and 9443 are separated by 184 nucleotides9443 and 9444 are separated by 248 nucleotides9444 and 9445 are separated by 395 nucleotides mRNA_1073: 9441 - Hypothetical Protein, at 343,845 to 345,302 9441 mRNA_1074: 9442 - HMMPfam-Ankyrin repeats (3 copies)-PF12796,ProSiteProfiles-Ankyrin repeat profile.-PS50088,ProSiteProfiles-Ankyrin repeat region circular profile.-PS50297,SUPERFAMILY--SSF48403, at 346,068 to 346,985 9442 mRNA_1075: 9443 - HMMPfam-F-box domain-PF00646,ProSiteProfiles-F-box domain profile.-PS50181,SUPERFAMILY--SSF81383, at 347,170 to 348,411 9443 mRNA_1076: 9444 - K02324 POLE1 DNA polymerase epsilon subunit 1, at 348,660 to 356,355 9444 mRNA_1077: 9445 - BLAST expressed protein [Cryptococcus neoformans var. neofo..., at 356,751 to 359,055 9445
Group Condition mRNA_1073 mRNA_1074 mRNA_1075 mRNA_1076 mRNA_1077
9441 9442 9443 9444 9445
auxotrophy Dropout Complete -1.0 -0.1 +0.1 N.D. -0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.3 -0.1 -0.3 N.D. -0.5
facs on lipid accumulation Sorted Low BODIPY Gate -0.5 -0.3 +0.0 N.D. -0.3
growth on oleic acid YNB Glucose -0.1 -0.4 -0.2 N.D. -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.6 -0.0 -0.1 N.D. -0.2
auxotrophy YNB Glucose 75mM Methionine -0.5 +0.2 -0.2 N.D. -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 -1.1 +0.3 N.D. -0.3
growth on oleic acid YNB Oleic Acid +0.1 -0.3 -0.0 N.D. -0.4
auxotrophy YNB Glucose 75mM Arginine -0.6 +0.0 +0.0 N.D. -0.1
auxotrophy YNB Glucose -0.6 +0.2 +0.0 N.D. -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 -0.4 -0.1 N.D. -0.0
growth on ricinoleic acid YNB Glucose +0.2 -0.7 +0.4 N.D. -0.3
ypd buoyancy 40 hrs High Buoyancy -0.4 -0.2 +0.0 N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.5 +0.0 +0.0 N.D. +0.1
growth on ypd YPD +0.4 -0.5 -0.1 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.1 -0.0 N.D. -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.0 +0.1 N.D. -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.7 +0.6 +0.3 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 +0.5 +0.1 N.D. -0.0
facs on lipid accumulation Sorted No BODIPY Gate +0.2 +0.0 +0.4 N.D. -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 +0.3 +0.4 N.D. -0.2
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