Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9442 and 9443 are separated by 184 nucleotides9443 and 9444 are separated by 248 nucleotides9444 and 9445 are separated by 395 nucleotides9445 and 9446 are separated by 798 nucleotides mRNA_1074: 9442 - HMMPfam-Ankyrin repeats (3 copies)-PF12796,ProSiteProfiles-Ankyrin repeat profile.-PS50088,ProSiteProfiles-Ankyrin repeat region circular profile.-PS50297,SUPERFAMILY--SSF48403, at 346,068 to 346,985 9442 mRNA_1075: 9443 - HMMPfam-F-box domain-PF00646,ProSiteProfiles-F-box domain profile.-PS50181,SUPERFAMILY--SSF81383, at 347,170 to 348,411 9443 mRNA_1076: 9444 - K02324 POLE1 DNA polymerase epsilon subunit 1, at 348,660 to 356,355 9444 mRNA_1077: 9445 - BLAST expressed protein [Cryptococcus neoformans var. neofo..., at 356,751 to 359,055 9445 mRNA_1078: 9446 - K00784 rnz ribonuclease Z, at 359,854 to 363,039 9446
Group Condition mRNA_1074 mRNA_1075 mRNA_1076 mRNA_1077 mRNA_1078
9442 9443 9444 9445 9446
growth on ricinoleic acid YNB Methylricinoleic Acid -1.1 +0.3 N.D. -0.3 N.D.
growth on oleic acid YNB Glucose -0.4 -0.2 N.D. -0.3 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -0.3 N.D. -0.5 N.D.
growth on ypd YPD -0.5 -0.1 N.D. -0.1 N.D.
growth on oleic acid YNB Oleic Acid -0.3 -0.0 N.D. -0.4 N.D.
growth on ricinoleic acid YNB Glucose -0.7 +0.4 N.D. -0.3 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 -0.1 N.D. -0.0 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 +0.0 N.D. -0.3 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 -0.1 N.D. -0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.2 -0.2 N.D. -0.3 N.D.
auxotrophy Dropout Complete -0.1 +0.1 N.D. -0.2 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -0.0 N.D. -0.2 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.2 +0.0 N.D. +0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.0 +0.0 N.D. -0.1 N.D.
auxotrophy YNB Glucose +0.2 +0.0 N.D. -0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.0 +0.1 N.D. -0.0 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.0 +0.0 N.D. +0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.0 +0.4 N.D. -0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 +0.4 N.D. -0.2 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 +0.1 N.D. -0.0 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.6 +0.3 N.D. -0.1 N.D.
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