Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9455 and 9456 are separated by 482 nucleotides9456 and 9457 are separated by 159 nucleotides9457 and 9458 are separated by 288 nucleotides9458 and 9459 are separated by 370 nucleotides mRNA_1087: 9455 - KOG2793 Putative N2,N2-dimethylguanosine tRNA methyltransferase, at 377,970 to 379,646 9455 mRNA_1088: 9456 - KOG3179 Predicted glutamine synthetase, at 380,129 to 381,362 9456 mRNA_1089: 9457 - K01638 aceB, glcB malate synthase, at 381,522 to 383,960 9457 mRNA_1090: 9458 - K12393 AP1M AP-1 complex subunit mu, at 384,249 to 386,320 9458 mRNA_1091: 9459 - Hypothetical Protein, at 386,691 to 388,371 9459
Group Condition mRNA_1087 mRNA_1088 mRNA_1089 mRNA_1090 mRNA_1091
9455 9456 9457 9458 9459
growth on oleic acid YNB Oleic Acid -0.2 -0.5 -2.9 N.D. +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.4 -3.0 N.D. +0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 +0.3 -2.6 N.D. +0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 -0.0 -0.2 N.D. +0.4
facs on lipid accumulation Sorted High BODIPY Gate -0.3 +0.0 +0.1 N.D. -0.2
ypd buoyancy 40 hrs High Buoyancy +0.2 -0.4 +0.1 N.D. -0.3
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.4 +0.2 N.D. -0.1
growth on oleic acid YNB Glucose -0.1 +0.1 -0.4 N.D. +0.1
auxotrophy YNB Glucose 75mM Arginine -0.3 +0.4 -0.2 N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine -0.3 +0.2 -0.2 N.D. +0.4
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.1 +0.1 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 -0.0 -0.1 N.D. +0.0
growth on ypd YPD +0.3 +0.2 -0.2 N.D. +0.1
auxotrophy YNB Glucose -0.2 +0.3 -0.1 N.D. +0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.1 +0.3 N.D. -0.1
growth on ricinoleic acid YNB Glucose -0.0 -0.1 +0.0 N.D. +0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 -0.1 +0.4 N.D. -0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 +0.7 -0.0 N.D. -0.4
auxotrophy Dropout Complete -0.0 +0.5 -0.1 N.D. +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 +0.4 +0.0 N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.3 +0.7 +0.2 N.D. +0.4
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