Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9467 and 9468 are separated by 286 nucleotides9468 and 9469 are separated by 269 nucleotides9469 and 9470 are separated by 168 nucleotides9470 and 9471 are separated by 581 nucleotides mRNA_1099: 9467 - K08515 VAMP7 vesicle-associated membrane protein 7, at 401,360 to 402,298 9467 mRNA_1100: 9468 - K14679 TRL1 tRNA ligase, at 402,585 to 405,523 9468 mRNA_1101: 9469 - KOG1206 Peroxisomal multifunctional beta-oxidation protein and related enzymes, at 405,793 to 407,272 9469 mRNA_1102: 9470 - K03327 TC.MATE, SLC47A, norM, mdtK, dinF multidrug resistance protein, MATE family, at 407,441 to 409,508 9470 mRNA_1103: 9471 - KOG0252 Inorganic phosphate transporter, at 410,090 to 412,457 9471
Group Condition mRNA_1099 mRNA_1100 mRNA_1101 mRNA_1102 mRNA_1103
9467 9468 9469 9470 9471
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 N.D. +0.3 -1.0 -1.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 N.D. -0.8 -0.2 -0.2
growth on oleic acid YNB Glucose -0.1 N.D. -0.3 +0.3 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 N.D. -0.2 -0.3 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 N.D. -0.6 -0.1 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. -0.0 -0.0 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. +0.1 -0.1 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 N.D. -0.4 +0.5 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.4 N.D. -0.3 +0.4 -0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.2 N.D. -0.4 +0.8 +0.3
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. -0.2 +0.4 -0.1
growth on oleic acid YNB Oleic Acid +0.0 N.D. +0.0 +0.4 +0.1
auxotrophy YNB Glucose +0.3 N.D. +0.0 +0.4 -0.1
growth on ricinoleic acid YNB Glucose +0.6 N.D. -0.2 +0.4 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. +0.1 -0.1 +0.5
auxotrophy Dropout Complete +0.5 N.D. -0.1 +0.7 -0.3
ypd buoyancy 40 hrs Low Buoyancy +0.9 N.D. +0.2 +0.3 -0.4
growth on ypd YPD +0.5 N.D. +0.1 +0.6 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.8 N.D. -0.1 +0.5 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 N.D. +0.1 +0.4 +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.2 N.D. -0.2 +0.6 +0.8
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