Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9510 and 9511 are separated by 362 nucleotides9511 and 9512 are separated by 104 nucleotides9512 and 9513 are separated by 772 nucleotides9513 and 9514 are separated by 449 nucleotides mRNA_1142: 9510 - K03139 TFIIF2, GTF2F2, TFG2 transcription initiation factor TFIIF subunit beta, at 503,587 to 505,304 9510 mRNA_1143: 9511 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 505,667 to 510,461 9511 mRNA_1144: 9512 - K13355 NUDT19 nucleoside diphosphate-linked moiety X motif 19, mitochondrial, at 510,566 to 511,961 9512 mRNA_1145: 9513 - K00669 AANAT arylalkylamine N-acetyltransferase, at 512,734 to 513,942 9513 mRNA_1146: 9514 - K06682 TEM1 Gtp-binding protein of the ras superfamily involved in termination of M-phase, at 514,392 to 515,549 9514
Group Condition mRNA_1142 mRNA_1143 mRNA_1144 mRNA_1145 mRNA_1146
9510 9511 9512 9513 9514
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.0 -0.9 -0.3 -0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.2 -0.2 -0.3 -0.0
growth on oleic acid YNB Oleic Acid N.D. -0.3 -0.2 -0.0 -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. -0.1 -0.4 +0.3 -0.3
growth on oleic acid YNB Glucose N.D. -0.2 -0.1 +0.3 -0.4
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.0 +0.0 -0.5 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.1 -0.1 -0.8 +0.5
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 -0.1 -0.1 -0.1
growth on ricinoleic acid YNB Glucose N.D. +0.0 +0.4 -0.3 -0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.1 +0.2 -0.3 -0.0
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 -0.1 -0.2 +0.3
auxotrophy YNB Glucose N.D. -0.0 -0.2 +0.2 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.1 +0.3 +0.1 -0.2
auxotrophy YNB Glucose 75mM Arginine N.D. -0.1 -0.1 +0.5 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.0 +0.5 -0.4 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 +0.5 -0.2 -0.2
auxotrophy Dropout Complete N.D. -0.2 -0.2 +0.8 -0.1
growth on ypd YPD N.D. -0.1 -0.0 +0.0 +0.6
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.0 +0.5 +0.1 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.1 +0.6 +0.0 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.0 +0.6 +0.5 +0.3
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