Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9527 and 9528 are separated by 32 nucleotides9528 and 9529 are separated by 444 nucleotides9529 and 9530 are separated by 403 nucleotides9530 and 9531 are separated by 442 nucleotides mRNA_1159: 9527 - HMMPfam-Archaeal ATPase-PF01637,SUPERFAMILY--SSF52540, at 538,557 to 540,883 9527 mRNA_1160: 9528 - KOG4159 Predicted E3 ubiquitin ligase, at 540,916 to 543,538 9528 mRNA_1161: 9529 - K03028 PSMD2, RPN1 26S proteasome regulatory subunit N1, at 543,983 to 547,395 9529 mRNA_1162: 9530 - SUPERFAMILY--SSF52047, at 547,799 to 548,923 9530 mRNA_1163: 9531 - KOG1378 Purple acid phosphatase, at 549,366 to 551,873 9531
Group Condition mRNA_1159 mRNA_1160 mRNA_1161 mRNA_1162 mRNA_1163
9527 9528 9529 9530 9531
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 -1.2 N.D. -0.7 -0.8
facs on lipid accumulation Sorted High BODIPY Gate -0.3 -1.0 N.D. -0.3 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 -0.5 N.D. +0.0 -0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.2 N.D. +0.1 -0.3
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.2 N.D. -0.1 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 -0.4 N.D. +0.2 -0.2
ypd buoyancy 40 hrs High Buoyancy -0.4 +0.0 N.D. -0.0 +0.2
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.0 N.D. -0.0 -0.1
growth on ypd YPD +0.0 -0.1 N.D. -0.1 -0.0
growth on oleic acid YNB Glucose +0.1 -0.4 N.D. +0.1 -0.0
growth on oleic acid YNB Oleic Acid +0.0 -0.1 N.D. -0.1 -0.1
auxotrophy YNB Glucose 75mM Methionine +0.2 -0.8 N.D. +0.3 +0.1
auxotrophy YNB Glucose 75mM Arginine -0.0 -0.4 N.D. +0.2 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 -0.1 N.D. -0.1 -0.0
auxotrophy YNB Glucose +0.2 -0.4 N.D. +0.3 +0.1
growth on ricinoleic acid YNB Glucose +0.2 -0.1 N.D. +0.3 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +0.3 N.D. -0.1 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 +0.1 N.D. +0.4 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 +0.7 N.D. -0.4 -0.0
auxotrophy Dropout Complete +0.3 -0.2 N.D. +0.3 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 +0.6 N.D. +0.1 +0.1
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