Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9533 and 9534 are separated by 415 nucleotides9534 and 9535 are separated by 526 nucleotides9535 and 9536 are separated by 223 nucleotides9536 and 9537 are separated by 523 nucleotides mRNA_1165: 9533 - HMMPfam-Protein of unknown function (DUF1445)-PF07286,PIRSF--PIRSF029755,SUPERFAMILY--SSF160920, at 553,391 to 554,372 9533 mRNA_1166: 9534 - KOG2533 Permease of the major facilitator superfamily, at 554,788 to 557,060 9534 mRNA_1167: 9535 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 557,587 to 560,660 9535 mRNA_1168: 9536 - K01474 hyuB N-methylhydantoinase B, at 560,884 to 563,023 9536 mRNA_1169: 9537 - K01469 OPLAH, OXP1, oplAH 5-oxoprolinase (ATP-hydrolysing), at 563,547 to 566,148 9537
Group Condition mRNA_1165 mRNA_1166 mRNA_1167 mRNA_1168 mRNA_1169
9533 9534 9535 9536 9537
ypd buoyancy 40 hrs No Buoyancy Separation -1.4 -0.4 -0.4 -0.1 -0.2
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.3 -0.0 -0.0 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -0.0 -0.5 +0.3 -0.5
growth on ricinoleic acid YNB Glucose -0.1 -0.3 +0.0 -0.2 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 -0.1 -0.3 -0.1 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -0.1 -0.1 +0.2 +0.0
facs on lipid accumulation Sorted No BODIPY Gate +0.2 +0.0 -0.2 +0.5 -0.4
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.2 +0.2 +0.2 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 +0.1 -0.1 +0.1 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.2 -0.2 +0.0 -0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.0 -0.0 -0.4 +0.7 +0.0
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.4 +0.3 +0.6 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 +0.1 -0.0 +0.3 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 +0.2 -0.1 +0.1 -0.1
growth on oleic acid YNB Oleic Acid +0.5 -0.4 +0.2 +0.2 -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.3 +0.0 +0.3 +0.1 -0.3
auxotrophy YNB Glucose +0.0 -0.2 +0.3 +0.6 -0.2
growth on oleic acid YNB Glucose +0.5 -0.1 +0.2 +0.4 -0.3
ypd buoyancy 40 hrs Low Buoyancy +0.2 +0.0 +0.4 +0.3 -0.1
auxotrophy Dropout Complete +0.4 -0.3 +0.5 +0.3 -0.1
growth on ypd YPD +0.1 +0.1 +0.3 +0.7 -0.1
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