Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9534 and 9535 are separated by 526 nucleotides9535 and 9536 are separated by 223 nucleotides9536 and 9537 are separated by 523 nucleotides9537 and 9538 are separated by 28 nucleotides mRNA_1166: 9534 - KOG2533 Permease of the major facilitator superfamily, at 554,788 to 557,060 9534 mRNA_1167: 9535 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 557,587 to 560,660 9535 mRNA_1168: 9536 - K01474 hyuB N-methylhydantoinase B, at 560,884 to 563,023 9536 mRNA_1169: 9537 - K01469 OPLAH, OXP1, oplAH 5-oxoprolinase (ATP-hydrolysing), at 563,547 to 566,148 9537 mRNA_1170: 9538 - K09531 DNAJC11 DnaJ homolog subfamily C member 11, at 566,177 to 568,431 9538
Group Condition mRNA_1166 mRNA_1167 mRNA_1168 mRNA_1169 mRNA_1170
9534 9535 9536 9537 9538
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 -0.5 +0.3 -0.5 -0.9
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 -0.4 -0.1 -0.2 +0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.0 -0.4 +0.7 +0.0 -1.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.3 -0.1 +0.1 -0.3
growth on ricinoleic acid YNB Glucose -0.3 +0.0 -0.2 +0.1 -0.4
ypd buoyancy 40 hrs High Buoyancy -0.3 -0.0 -0.0 -0.3 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 -0.1 +0.1 -0.1 -0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 -0.2 +0.0 -0.0 -0.5
facs on lipid accumulation Sorted No BODIPY Gate +0.0 -0.2 +0.5 -0.4 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 -0.1 +0.1 -0.1 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -0.1 +0.2 +0.0 -0.1
growth on oleic acid YNB Oleic Acid -0.4 +0.2 +0.2 -0.2 +0.0
ypd buoyancy 40 hrs Median Buoyancy +0.0 +0.3 +0.1 -0.3 -0.2
growth on oleic acid YNB Glucose -0.1 +0.2 +0.4 -0.3 -0.2
auxotrophy YNB Glucose 75mM Methionine -0.2 +0.2 +0.2 -0.1 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -0.0 +0.3 +0.1 -0.3
ypd buoyancy 40 hrs Low Buoyancy +0.0 +0.4 +0.3 -0.1 -0.4
auxotrophy YNB Glucose 75mM Arginine -0.4 +0.3 +0.6 -0.2 +0.2
auxotrophy YNB Glucose -0.2 +0.3 +0.6 -0.2 +0.4
auxotrophy Dropout Complete -0.3 +0.5 +0.3 -0.1 +0.5
growth on ypd YPD +0.1 +0.3 +0.7 -0.1 +0.3
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