Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9536 and 9537 are separated by 523 nucleotides9537 and 9538 are separated by 28 nucleotides9538 and 9539 are separated by 120 nucleotides9539 and 9540 are separated by 372 nucleotides mRNA_1168: 9536 - K01474 hyuB N-methylhydantoinase B, at 560,884 to 563,023 9536 mRNA_1169: 9537 - K01469 OPLAH, OXP1, oplAH 5-oxoprolinase (ATP-hydrolysing), at 563,547 to 566,148 9537 mRNA_1170: 9538 - K09531 DNAJC11 DnaJ homolog subfamily C member 11, at 566,177 to 568,431 9538 mRNA_1171: 9539 - K07277 SAM50, TOB55, bamA outer membrane protein insertion porin family, at 568,552 to 570,961 9539 mRNA_1172: 9540 - KOG2277 S-M checkpoint control protein CID1 and related nucleotidyltransferases, at 571,334 to 573,898 9540
Group Condition mRNA_1168 mRNA_1169 mRNA_1170 mRNA_1171 mRNA_1172
9536 9537 9538 9539 9540
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 -0.5 -0.9 N.D. +0.1
growth on ricinoleic acid YNB Glucose -0.2 +0.1 -0.4 N.D. -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 -0.2 +0.2 N.D. -0.5
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 +0.1 -0.3 N.D. -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.3 -0.2 N.D. +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 -0.0 -0.5 N.D. +0.2
ypd buoyancy 40 hrs High Buoyancy -0.0 -0.3 -0.1 N.D. +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.7 +0.0 -1.0 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -0.1 -0.6 N.D. +0.4
ypd buoyancy 40 hrs Low Buoyancy +0.3 -0.1 -0.4 N.D. -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 -0.1 -0.2 N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.5 -0.4 -0.2 N.D. +0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 +0.0 -0.1 N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine +0.2 -0.1 +0.1 N.D. +0.1
growth on oleic acid YNB Oleic Acid +0.2 -0.2 +0.0 N.D. +0.2
growth on oleic acid YNB Glucose +0.4 -0.3 -0.2 N.D. +0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 +0.1 -0.3 N.D. +0.4
auxotrophy YNB Glucose 75mM Arginine +0.6 -0.2 +0.2 N.D. +0.0
auxotrophy YNB Glucose +0.6 -0.2 +0.4 N.D. +0.0
auxotrophy Dropout Complete +0.3 -0.1 +0.5 N.D. +0.0
growth on ypd YPD +0.7 -0.1 +0.3 N.D. +0.3
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