Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9542 and 9543 are separated by 484 nucleotides9543 and 9544 are separated by 229 nucleotides9544 and 9545 are separated by 590 nucleotides9545 and 9546 are separated by 161 nucleotides mRNA_1174: 9542 - K15190 MEPCE, BCDIN3 7SK snRNA methylphosphate capping enzyme, at 575,723 to 577,057 9542 mRNA_1175: 9543 - Hypothetical Protein, at 577,542 to 578,636 9543 mRNA_1176: 9544 - Hypothetical Protein, at 578,866 to 579,597 9544 mRNA_1177: 9545 - K15326 TSEN54 tRNA-splicing endonuclease subunit Sen54, at 580,188 to 581,963 9545 mRNA_1178: 9546 - Hypothetical Protein, at 582,125 to 583,749 9546
Group Condition mRNA_1174 mRNA_1175 mRNA_1176 mRNA_1177 mRNA_1178
9542 9543 9544 9545 9546
growth on oleic acid YNB Oleic Acid +0.3 -0.0 -0.4 N.D. -0.7
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 -0.1 -0.2 N.D. +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -0.0 -0.5 N.D. +0.3
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.1 -0.1 N.D. -0.1
growth on ricinoleic acid YNB Glucose -0.2 -0.0 +0.0 N.D. +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 -0.3 +0.6 N.D. -0.3
growth on ypd YPD +0.1 -0.2 -0.1 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 -0.2 -0.0 N.D. +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 +0.1 +0.1 N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.0 +0.2 -0.2 N.D. +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.2 +0.5 N.D. -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.4 -0.4 +0.7 N.D. +0.3
auxotrophy YNB Glucose 75mM Methionine +0.0 -0.1 +0.0 N.D. +0.4
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.1 +0.1 N.D. +0.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 +0.2 +0.1 N.D. -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.2 -0.2 N.D. +0.2
growth on oleic acid YNB Glucose +0.5 +0.3 -0.1 N.D. -0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.4 +0.0 -0.3 N.D. +0.4
auxotrophy YNB Glucose -0.0 -0.1 +0.0 N.D. +0.8
auxotrophy Dropout Complete -0.3 +0.1 -0.0 N.D. +1.0
auxotrophy YNB Glucose 75mM Arginine -0.0 -0.2 +0.1 N.D. +1.0
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