Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9545 and 9546 are separated by 161 nucleotides9546 and 9547 are separated by 265 nucleotides9547 and 9548 are separated by 780 nucleotides9548 and 9549 are separated by 283 nucleotides mRNA_1177: 9545 - K15326 TSEN54 tRNA-splicing endonuclease subunit Sen54, at 580,188 to 581,963 9545 mRNA_1178: 9546 - Hypothetical Protein, at 582,125 to 583,749 9546 mRNA_1179: 9547 - K12611 DCP1B mRNA-decapping enzyme 1B, at 584,015 to 586,277 9547 mRNA_1180: 9548 - Hypothetical Protein, at 587,058 to 592,378 9548 mRNA_1181: 9549 - Hypothetical Protein, at 592,662 to 593,327 9549
Group Condition mRNA_1177 mRNA_1178 mRNA_1179 mRNA_1180 mRNA_1181
9545 9546 9547 9548 9549
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.1 -1.0 -0.2 -1.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.3 -1.3 -0.1 -0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.4 -0.2 -0.1 -1.6
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 -0.2 +0.0 -0.9
growth on ricinoleic acid YNB Glucose N.D. +0.2 +0.2 +0.0 -1.4
auxotrophy Dropout Complete N.D. +1.0 -0.4 +0.0 -1.5
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.2 -0.4 -0.2 -0.5
auxotrophy YNB Glucose 75mM Methionine N.D. +0.4 -0.0 +0.1 -1.3
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.2 -0.5 -0.1 -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.0 -0.4 +0.1 -0.4
growth on oleic acid YNB Glucose N.D. -0.3 +0.2 -0.2 -0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 -0.5 -0.0 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 -0.2 +0.0 -0.5
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.1 -0.1 +0.1 -0.6
auxotrophy YNB Glucose N.D. +0.8 -0.2 +0.2 -1.2
growth on oleic acid YNB Oleic Acid N.D. -0.7 +0.7 -0.0 -0.3
auxotrophy YNB Glucose 75mM Arginine N.D. +1.0 +0.1 +0.1 -0.9
growth on ypd YPD N.D. +0.2 +0.4 -0.1 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.3 +0.3 +0.3 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.3 +0.2 -0.0 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.3 +0.6 -0.0 +0.0
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