Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9550 and 9551 are separated by 267 nucleotides9551 and 9552 are separated by 558 nucleotides9552 and 9553 are separated by 432 nucleotides9553 and 9554 are separated by 96 nucleotides mRNA_1182: 9550 - K12816 CDC40, PRP17 pre-mRNA-processing factor 17, at 593,740 to 596,442 9550 mRNA_1183: 9551 - KOG2533 Permease of the major facilitator superfamily, at 596,710 to 598,781 9551 mRNA_1184: 9552 - KOG0143 Iron/ascorbate family oxidoreductases, at 599,340 to 600,971 9552 mRNA_1185: 9553 - KOG1399 Flavin-containing monooxygenase, at 601,404 to 603,765 9553 mRNA_1186: 9554 - K20410 MAPKAP1, SIN1 target of rapamycin complex 2 subunit MAPKAP1, at 603,862 to 607,168 9554
Group Condition mRNA_1182 mRNA_1183 mRNA_1184 mRNA_1185 mRNA_1186
9550 9551 9552 9553 9554
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.1 -0.1 +0.1 -1.7
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.2 -0.2 +0.2 -1.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.4 +0.0 +0.3 -0.6
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.3 -0.1 +0.0 -0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 -0.2 -0.1 -0.2
growth on oleic acid YNB Oleic Acid N.D. -0.1 +0.0 -0.1 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.3 -0.1 -0.0 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. +0.0 -0.1 -0.3 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.3 +0.1 -0.2 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.2 +0.0 +0.0 +0.1
auxotrophy Dropout Complete N.D. +0.0 +0.1 -0.2 -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.1 -0.0 +0.0 -0.2
auxotrophy YNB Glucose 75mM Arginine N.D. +0.0 -0.0 -0.1 +0.1
auxotrophy YNB Glucose N.D. +0.1 -0.1 -0.0 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.2 +0.2 -0.1 +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 +0.2 -0.1 -0.0
growth on oleic acid YNB Glucose N.D. +0.0 -0.0 +0.0 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.0 -0.0 +0.0 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. +0.0 +0.1 -0.0 +0.1
growth on ricinoleic acid YNB Glucose N.D. +0.1 +0.2 +0.0 +0.1
growth on ypd YPD N.D. +0.3 +0.1 +0.3 -0.1
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