Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9553 and 9554 are separated by 96 nucleotides9554 and 9555 are separated by 290 nucleotides9555 and 9556 are separated by 271 nucleotides9556 and 9557 are separated by 573 nucleotides mRNA_1185: 9553 - KOG1399 Flavin-containing monooxygenase, at 601,404 to 603,765 9553 mRNA_1186: 9554 - K20410 MAPKAP1, SIN1 target of rapamycin complex 2 subunit MAPKAP1, at 603,862 to 607,168 9554 mRNA_1187: 9555 - Hypothetical Protein, at 607,459 to 608,635 9555 mRNA_1188: 9556 - Hypothetical Protein, at 608,907 to 610,370 9556 mRNA_1189: 9557 - K02183 CALM calmodulin, at 610,944 to 612,445 9557
Group Condition mRNA_1185 mRNA_1186 mRNA_1187 mRNA_1188 mRNA_1189
9553 9554 9555 9556 9557
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -1.3 -0.6 -1.4 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -1.7 -0.9 +0.3 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 +0.2 -0.7 -1.3 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.0 -0.2 -0.0 -0.9 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.0 +0.0 -0.9 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.2 -0.2 -0.2 N.D.
growth on oleic acid YNB Oleic Acid -0.1 -0.2 +0.0 -0.2 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.3 -0.6 -0.2 +0.0 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 +0.1 +0.3 -0.5 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.0 +0.1 +0.1 -0.2 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 -0.7 +0.1 +0.6 N.D.
growth on oleic acid YNB Glucose +0.0 +0.1 +0.1 -0.2 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 +0.2 +0.1 -0.0 N.D.
growth on ricinoleic acid YNB Glucose +0.0 +0.1 -0.1 +0.3 N.D.
auxotrophy Dropout Complete -0.2 -0.1 +0.1 +0.5 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 -0.1 +0.2 +0.5 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.3 +0.1 -0.1 +0.9 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.1 +0.1 +0.3 +0.6 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 +0.1 +0.0 +1.0 N.D.
growth on ypd YPD +0.3 -0.1 -0.1 +1.0 N.D.
auxotrophy YNB Glucose -0.0 +0.0 +0.3 +1.3 N.D.
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