Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9557 and 9558 are separated by 612 nucleotides9558 and 9559 are separated by 400 nucleotides9559 and 9560 are separated by 130 nucleotides9560 and 9561 are separated by 314 nucleotides mRNA_1189: 9557 - K02183 CALM calmodulin, at 610,944 to 612,445 9557 mRNA_1190: 9558 - K05747 WAS Wiskott-Aldrich syndrome protein, at 613,058 to 614,661 9558 mRNA_1191: 9559 - KOG3033 Predicted PhzC/PhzF-type epimerase, at 615,062 to 616,261 9559 mRNA_1192: 9560 - KOG2504 Monocarboxylate transporter, at 616,392 to 618,414 9560 mRNA_1193: 9561 - KOG2504 Monocarboxylate transporter, at 618,729 to 620,711 9561
Group Condition mRNA_1189 mRNA_1190 mRNA_1191 mRNA_1192 mRNA_1193
9557 9558 9559 9560 9561
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.7 -0.3 -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.1 -0.2 -0.4
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.1 +0.1 -0.3
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.1 -0.1 -0.2
growth on oleic acid YNB Glucose N.D. N.D. +0.1 -0.1 -0.2
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.3 -0.2 -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.3 -0.2 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.4 -0.2 -0.2
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.1 +0.0 -0.1
auxotrophy Dropout Complete N.D. N.D. +0.1 -0.1 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.3 -0.2 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.1 -0.1 +0.3
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +0.1 +0.1 -0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.1 -0.0 +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.1 +0.0 +0.0
auxotrophy YNB Glucose N.D. N.D. +0.3 -0.0 +0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.4 -0.0 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.3 +0.1 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.1 +0.1 +0.3
growth on ypd YPD N.D. N.D. +0.3 +0.3 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.2 +0.2 +0.3
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