Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9561 and 9562 are separated by 441 nucleotides9562 and 9563 are separated by 469 nucleotides9563 and 9564 are separated by 792 nucleotides9564 and 9565 are separated by 1287 nucleotides mRNA_1193: 9561 - KOG2504 Monocarboxylate transporter, at 618,729 to 620,711 9561 mRNA_1194: 9562 - K05925 METTL3_14 mRNA (2'-O-methyladenosine-N6-)-methyltransferase, at 621,153 to 623,607 9562 mRNA_1195: 9563 - K03248 EIF3G translation initiation factor 3 subunit G, at 624,077 to 625,434 9563 mRNA_1196: 9564 - HMMPfam-Membrane bound O-acyl transferase family-PF13813, at 626,227 to 627,477 9564 mRNA_1197: 9565 - KOG2385 Uncharacterized conserved protein, at 628,765 to 631,426 9565
Group Condition mRNA_1193 mRNA_1194 mRNA_1195 mRNA_1196 mRNA_1197
9561 9562 9563 9564 9565
auxotrophy YNB Glucose +0.0 +0.4 N.D. -0.9 -0.6
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 -0.1 N.D. -0.3 -0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.3 -0.1 N.D. -0.8 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.4 -0.2 N.D. -0.7 -0.6
growth on oleic acid YNB Oleic Acid -0.1 +0.2 N.D. -0.8 -0.2
auxotrophy YNB Glucose 75mM Arginine -0.0 +0.3 N.D. -0.5 -0.7
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.0 N.D. -0.6 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 -0.1 N.D. -0.7 +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.2 N.D. -0.5 -0.3
auxotrophy YNB Glucose 75mM Methionine -0.2 +0.1 N.D. -0.2 -0.1
growth on ricinoleic acid YNB Glucose -0.1 +0.5 N.D. -0.4 -0.4
auxotrophy Dropout Complete +0.0 +0.4 N.D. -0.2 -0.4
growth on oleic acid YNB Glucose -0.2 +0.4 N.D. -0.2 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.0 +0.1 N.D. -0.1 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.3 N.D. +0.4 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 +0.8 N.D. -0.9 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 +0.5 N.D. +0.1 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 -0.3 N.D. +0.5 +0.2
growth on ypd YPD +0.2 -0.2 N.D. +0.3 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 +0.6 N.D. -0.3 +0.5
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 +0.6 N.D. +0.1 +0.7
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