Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9567 and 9568 are separated by 212 nucleotides9568 and 9569 are separated by 201 nucleotides9569 and 9570 are separated by 1255 nucleotides9570 and 9571 are separated by 688 nucleotides mRNA_1199: 9567 - K02325 POLE2 DNA polymerase epsilon subunit 2, at 634,120 to 636,519 9567 mRNA_1200: 9568 - KOG3132 m3G-cap-specific nuclear import receptor (Snurportin1), at 636,732 to 638,216 9568 mRNA_1201: 9569 - K12625 LSM6 U6 snRNA-associated Sm-like protein LSm6, at 638,418 to 638,898 9569 mRNA_1202: 9570 - K11253 H3 histone H3, at 640,154 to 640,968 9570 mRNA_1203: 9571 - K01049 ACHE acetylcholinesterase, at 641,657 to 643,946 9571
Group Condition mRNA_1199 mRNA_1200 mRNA_1201 mRNA_1202 mRNA_1203
9567 9568 9569 9570 9571
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -1.1 N.D. -1.8 -0.5
ypd buoyancy 40 hrs High Buoyancy N.D. -0.3 N.D. -1.5 +0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.6 N.D. -0.4 -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.0 N.D. -0.7 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.1 N.D. -0.9 +0.1
auxotrophy Dropout Complete N.D. +0.2 N.D. -0.2 -0.3
growth on ypd YPD N.D. -0.0 N.D. -0.1 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.3 N.D. +0.3 +0.0
auxotrophy YNB Glucose 75mM Methionine N.D. +0.1 N.D. +0.2 -0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.0 N.D. +0.2 -0.0
auxotrophy YNB Glucose 75mM Arginine N.D. -0.1 N.D. +0.5 -0.1
growth on oleic acid YNB Glucose N.D. +0.2 N.D. +0.2 -0.1
growth on oleic acid YNB Oleic Acid N.D. -0.0 N.D. +0.1 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.7 N.D. -0.5 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.1 N.D. +0.7 -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.3 N.D. +0.1 +0.1
auxotrophy YNB Glucose N.D. +0.1 N.D. +0.5 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.0 N.D. +0.8 -0.2
growth on ricinoleic acid YNB Glucose N.D. +0.8 N.D. -0.2 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.2 N.D. +0.8 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.0 N.D. +1.2 -0.2
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