Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9576 and 9577 are separated by 781 nucleotides9577 and 9578 are separated by 246 nucleotides9578 and 9579 are separated by 160 nucleotides9579 and 9580 are separated by 640 nucleotides mRNA_1208: 9576 - Hypothetical Protein, at 654,307 to 655,456 9576 mRNA_1209: 9577 - Hypothetical Protein, at 656,238 to 657,706 9577 mRNA_1210: 9578 - K13076 SLD Delta8-fatty-acid desaturase, at 657,953 to 659,955 9578 mRNA_1211: 9579 - K15078 SLX1 structure-specific endonuclease subunit SLX1, at 660,116 to 662,467 9579 mRNA_1212: 9580 - HMMPfam-Regulator of G protein signaling domain-PF00615,ProSiteProfiles-RGS domain profile.-PS50132,SMART-Regulator of G protein signalling domain-SM00315,SUPERFAMILY--SSF48097, at 663,108 to 664,692 9580
Group Condition mRNA_1208 mRNA_1209 mRNA_1210 mRNA_1211 mRNA_1212
9576 9577 9578 9579 9580
facs on lipid accumulation Sorted High BODIPY Gate -2.2 N.D. N.D. +0.4 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.5 N.D. N.D. -0.1 N.D.
growth on oleic acid YNB Glucose -0.5 N.D. N.D. +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.4 N.D. N.D. +0.1 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 N.D. N.D. -0.0 N.D.
growth on ricinoleic acid YNB Glucose -0.2 N.D. N.D. +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.5 N.D. N.D. +0.4 N.D.
growth on oleic acid YNB Oleic Acid +0.1 N.D. N.D. -0.2 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 N.D. N.D. +0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. N.D. +0.3 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.1 N.D. N.D. +0.2 N.D.
growth on ypd YPD +0.3 N.D. N.D. -0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 N.D. N.D. +0.2 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. N.D. +0.3 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.2 N.D. N.D. +0.5 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.2 N.D. N.D. +0.6 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 N.D. N.D. +0.4 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. N.D. +0.9 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 N.D. N.D. +0.6 N.D.
auxotrophy YNB Glucose +0.5 N.D. N.D. +0.9 N.D.
auxotrophy Dropout Complete +0.2 N.D. N.D. +1.2 N.D.
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