Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9577 and 9578 are separated by 246 nucleotides9578 and 9579 are separated by 160 nucleotides9579 and 9580 are separated by 640 nucleotides9580 and 9581 are separated by 396 nucleotides mRNA_1209: 9577 - Hypothetical Protein, at 656,238 to 657,706 9577 mRNA_1210: 9578 - K13076 SLD Delta8-fatty-acid desaturase, at 657,953 to 659,955 9578 mRNA_1211: 9579 - K15078 SLX1 structure-specific endonuclease subunit SLX1, at 660,116 to 662,467 9579 mRNA_1212: 9580 - HMMPfam-Regulator of G protein signaling domain-PF00615,ProSiteProfiles-RGS domain profile.-PS50132,SMART-Regulator of G protein signalling domain-SM00315,SUPERFAMILY--SSF48097, at 663,108 to 664,692 9580 mRNA_1213: 9581 - KOG1205 Predicted dehydrogenase, at 665,089 to 665,972 9581
Group Condition mRNA_1209 mRNA_1210 mRNA_1211 mRNA_1212 mRNA_1213
9577 9578 9579 9580 9581
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.2 N.D. -0.6
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.1 N.D. -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.1 N.D. -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.2 N.D. -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.0 N.D. -0.0
growth on oleic acid YNB Glucose N.D. N.D. +0.1 N.D. +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.6 N.D. -0.5
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.4 N.D. -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.4 N.D. -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.5 N.D. -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.3 N.D. +0.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.4 N.D. -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.2 N.D. +0.3
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.3 N.D. +0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.2 N.D. +0.3
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.1 N.D. +0.5
auxotrophy YNB Glucose N.D. N.D. +0.9 N.D. +0.3
growth on ypd YPD N.D. N.D. -0.1 N.D. +1.7
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +0.9 N.D. +0.9
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.6 N.D. +1.3
auxotrophy Dropout Complete N.D. N.D. +1.2 N.D. +0.7
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