Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9579 and 9580 are separated by 640 nucleotides9580 and 9581 are separated by 396 nucleotides9581 and 9582 are separated by 271 nucleotides9582 and 9583 are separated by 348 nucleotides mRNA_1211: 9579 - K15078 SLX1 structure-specific endonuclease subunit SLX1, at 660,116 to 662,467 9579 mRNA_1212: 9580 - HMMPfam-Regulator of G protein signaling domain-PF00615,ProSiteProfiles-RGS domain profile.-PS50132,SMART-Regulator of G protein signalling domain-SM00315,SUPERFAMILY--SSF48097, at 663,108 to 664,692 9580 mRNA_1213: 9581 - KOG1205 Predicted dehydrogenase, at 665,089 to 665,972 9581 mRNA_1214: 9582 - Hypothetical Protein, at 666,244 to 666,423 9582 mRNA_1215: 9583 - Hypothetical Protein, at 666,772 to 668,801 9583
Group Condition mRNA_1211 mRNA_1212 mRNA_1213 mRNA_1214 mRNA_1215
9579 9580 9581 9582 9583
growth on oleic acid YNB Oleic Acid -0.2 N.D. -0.6 -0.9 -0.4
ypd buoyancy 40 hrs High Buoyancy +0.3 N.D. +0.2 -1.2 -0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.4 N.D. -0.1 -0.4 -0.5
facs on lipid accumulation Sorted No BODIPY Gate +0.2 N.D. +0.3 -1.1 +0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 N.D. -0.2 +0.8 -1.2
growth on oleic acid YNB Glucose +0.1 N.D. +0.0 +0.1 -0.3
ypd buoyancy 40 hrs Low Buoyancy +0.5 N.D. -0.2 +0.2 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 N.D. -0.4 -0.3 +1.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 N.D. -0.0 +0.7 +0.3
ypd buoyancy 40 hrs Median Buoyancy +0.3 N.D. +0.0 +0.7 +0.1
auxotrophy YNB Glucose +0.9 N.D. +0.3 +0.6 -0.1
growth on ricinoleic acid YNB Glucose +0.1 N.D. +0.5 +1.1 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 N.D. -0.3 +0.3 +1.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. -0.1 +1.1 +1.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. -0.3 +1.5 +0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.6 N.D. -0.5 +1.4 +0.6
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. +0.3 +0.3 +1.6
auxotrophy Dropout Complete +1.2 N.D. +0.7 +0.2 +0.5
auxotrophy YNB Glucose 75mM Arginine +0.9 N.D. +0.9 +0.8 +0.4
auxotrophy YNB Glucose 75mM Methionine +0.6 N.D. +1.3 +0.3 +0.8
growth on ypd YPD -0.1 N.D. +1.7 -1.2 +2.6
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