Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9619 and 9620 are separated by 239 nucleotides9620 and 9621 are separated by 399 nucleotides9621 and 9622 are separated by 715 nucleotides9622 and 9623 are separated by 183 nucleotides mRNA_1251: 9619 - HMMPfam-Protein of unknown function (DUF2611)-PF11022, at 756,531 to 757,355 9619 mRNA_1252: 9620 - BLAST glycosyltransferase family 22 protein [Mixia osmu..., at 757,595 to 759,040 9620 mRNA_1253: 9621 - K04554 UBE2J2, NCUBE2, UBC6 ubiquitin-conjugating enzyme E2 J2, at 759,440 to 760,414 9621 mRNA_1254: 9622 - K02150 ATPeV1E, ATP6E V-type H+-transporting ATPase subunit E, at 761,130 to 762,354 9622 mRNA_1255: 9623 - KOG1808 AAA ATPase containing von Willebrand factor type A (vWA) domain, at 762,538 to 767,686 9623
Group Condition mRNA_1251 mRNA_1252 mRNA_1253 mRNA_1254 mRNA_1255
9619 9620 9621 9622 9623
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 -0.2 -1.5 N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy -0.2 +0.2 -0.2 N.D. -0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 +0.2 -0.1 N.D. -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.7 +0.3 +0.3 N.D. -0.1
growth on ricinoleic acid YNB Glucose -0.5 +0.2 +0.3 N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.2 -0.1 -0.2 N.D. -0.0
auxotrophy YNB Glucose 75mM Arginine -0.1 +0.2 -0.0 N.D. -0.1
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.3 -0.1 N.D. -0.0
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.2 -0.3 N.D. +0.0
growth on ypd YPD +0.1 -0.3 +0.3 N.D. -0.1
auxotrophy YNB Glucose 75mM Methionine +0.0 +0.0 +0.3 N.D. -0.1
auxotrophy YNB Glucose -0.2 +0.2 +0.5 N.D. -0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.3 +0.1 N.D. +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 +0.0 +0.7 N.D. +0.0
auxotrophy Dropout Complete +0.4 +0.1 +0.2 N.D. -0.1
growth on oleic acid YNB Glucose -0.0 +0.2 +0.4 N.D. +0.0
growth on oleic acid YNB Oleic Acid +0.2 +0.2 +0.4 N.D. +0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 +0.2 +0.8 N.D. -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 +0.3 +1.0 N.D. +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +0.1 +1.3 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 +0.2 +1.5 N.D. +0.1
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