Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9626 and 9627 are separated by 425 nucleotides9627 and 9628 are separated by 201 nucleotides9628 and 9629 are separated by 1370 nucleotides9629 and 9630 are separated by 254 nucleotides mRNA_1258: 9626 - Hypothetical Protein, at 770,373 to 771,317 9626 mRNA_1259: 9627 - K10887 DCLRE1C, ARTEMIS, SCIDA DNA cross-link repair 1C protein, at 771,743 to 774,831 9627 mRNA_1260: 9628 - Hypothetical Protein, at 775,033 to 777,519 9628 mRNA_1261: 9629 - K04712 DEGS sphingolipid Delta-4 desaturase, at 778,890 to 780,707 9629 mRNA_1262: 9630 - KOG4781 Uncharacterized conserved protein, at 780,962 to 782,100 9630
Group Condition mRNA_1258 mRNA_1259 mRNA_1260 mRNA_1261 mRNA_1262
9626 9627 9628 9629 9630
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.3 +0.9 -4.3 -0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.3 +0.8 -3.1 -0.4
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.1 -0.9 -0.2 -0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.1 +0.5 -1.0 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.2 -0.7 +0.4 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.4 +0.9 -0.8 -0.5
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 -0.1 +0.1 +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. +0.2 +0.2 -0.3 +0.2
growth on ricinoleic acid YNB Glucose N.D. +0.2 +0.6 -0.8 +0.3
growth on oleic acid YNB Glucose N.D. +0.3 +0.8 -0.5 -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.2 +0.3 +0.1 +0.1
auxotrophy YNB Glucose 75mM Arginine N.D. +0.4 +0.8 -0.0 -0.3
auxotrophy YNB Glucose N.D. +0.4 +0.9 -0.1 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 +0.8 +0.6 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 +0.6 +1.0 -0.1
growth on ypd YPD N.D. +0.1 +0.4 +1.1 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.4 +1.1 +1.2 -0.1
growth on oleic acid YNB Oleic Acid N.D. +0.4 +1.4 +1.2 -0.2
auxotrophy Dropout Complete N.D. +0.4 +1.0 +1.9 -0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.3 +0.5 +1.6 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.4 +1.0 +1.9 +0.5
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