Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9658 and 9659 are separated by 112 nucleotides9659 and 9660 are separated by 167 nucleotides9660 and 9661 are separated by 438 nucleotides9661 and 9662 are separated by 831 nucleotides mRNA_1290: 9658 - K03872 TCEB1 transcription elongation factor B, polypeptide 1, at 844,469 to 844,987 9658 mRNA_1291: 9659 - KOG3350 Uncharacterized conserved protein, at 845,100 to 846,215 9659 mRNA_1292: 9660 - K10848 ERCC4, XPF DNA excision repair protein ERCC-4, at 846,383 to 850,419 9660 mRNA_1293: 9661 - Hypothetical Protein, at 850,858 to 851,999 9661 mRNA_1294: 9662 - K20369 ERV29, SURF4 ER-derived vesicles protein, at 852,831 to 853,984 9662
Group Condition mRNA_1290 mRNA_1291 mRNA_1292 mRNA_1293 mRNA_1294
9658 9659 9660 9661 9662
facs on lipid accumulation Sorted Low BODIPY Gate -1.5 N.D. -1.2 -0.1 -2.4
ypd buoyancy 40 hrs No Buoyancy Separation -2.8 N.D. +0.3 +0.1 -1.3
facs on lipid accumulation Sorted High BODIPY Gate -0.5 N.D. -0.8 -0.0 -1.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -2.0 N.D. +0.1 -0.3 +0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.0 N.D. -1.3 +0.2 -0.9
growth on ypd YPD -0.2 N.D. +0.2 -0.5 -0.5
ypd buoyancy 40 hrs High Buoyancy +0.3 N.D. -0.6 +0.2 -0.8
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 N.D. -0.3 -0.3 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. +0.0 -0.3 -0.4
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. -0.0 -0.4 +0.0
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. +0.2 +0.2 -0.4
auxotrophy YNB Glucose +0.6 N.D. +0.1 -0.6 -0.1
ypd buoyancy 40 hrs Low Buoyancy -0.3 N.D. -0.0 +0.3 +0.0
growth on oleic acid YNB Oleic Acid +0.5 N.D. -0.2 +0.1 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid -0.9 N.D. -0.1 +0.3 +0.7
auxotrophy YNB Glucose 75mM Arginine +0.4 N.D. +0.2 -0.5 +0.0
growth on oleic acid YNB Glucose +0.3 N.D. -0.0 +0.1 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 N.D. -0.2 -0.2 +0.9
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.7 N.D. -0.2 -0.3 +0.3
growth on ricinoleic acid YNB Glucose +0.9 N.D. +0.2 -0.2 +0.2
auxotrophy Dropout Complete +1.7 N.D. +0.3 -0.7 +0.3
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