Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9663 and 9664 are separated by 339 nucleotides9664 and 9665 are separated by 521 nucleotides9665 and 9666 are separated by 302 nucleotides9666 and 9667 are separated by 510 nucleotides mRNA_1295: 9663 - Hypothetical Protein, at 855,007 to 856,078 9663 mRNA_1296: 9664 - K19703 FA2H, SCS7 4-hydroxysphinganine ceramide fatty acyl 2-hydroxylase, at 856,418 to 857,945 9664 mRNA_1297: 9665 - K17292 TBCA tubulin-specific chaperone A, at 858,467 to 858,877 9665 mRNA_1298: 9666 - HMMPfam-Ubiquitin-2 like Rad60 SUMO-like-PF13881,ProSiteProfiles-Ubiquitin domain profile.-PS50053,SUPERFAMILY--SSF54236, at 859,180 to 859,998 9666 mRNA_1299: 9667 - K00600 glyA, SHMT glycine hydroxymethyltransferase, at 860,509 to 862,713 9667
Group Condition mRNA_1295 mRNA_1296 mRNA_1297 mRNA_1298 mRNA_1299
9663 9664 9665 9666 9667
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. -1.3 -0.2 -5.6
auxotrophy YNB Glucose 75mM Arginine +0.4 N.D. -0.5 -0.2 -5.7
auxotrophy YNB Glucose +0.3 N.D. -0.4 -0.0 -5.8
facs on lipid accumulation Sorted High BODIPY Gate +0.6 N.D. -1.1 -0.2 -4.5
auxotrophy YNB Glucose 75mM Methionine +0.4 N.D. -0.5 -0.1 -4.8
facs on lipid accumulation Sorted No BODIPY Gate +0.4 N.D. -0.3 +0.1 -4.6
growth on oleic acid YNB Glucose -0.4 N.D. +0.1 -0.1 -3.8
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 N.D. -0.6 -0.2 -3.1
growth on ricinoleic acid YNB Glucose +0.4 N.D. -0.3 -0.1 -3.8
auxotrophy Dropout Complete +0.7 N.D. +0.1 -0.2 -3.7
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. -0.0 -0.1 -2.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 N.D. -0.2 -0.0 -2.2
growth on oleic acid YNB Oleic Acid +0.2 N.D. -0.2 -0.1 -0.9
growth on ricinoleic acid YNB Ricinoleic Acid +0.6 N.D. +0.5 -0.2 -2.0
growth on ypd YPD +0.5 N.D. +1.3 -0.2 -2.5
ypd buoyancy 40 hrs Low Buoyancy +0.0 N.D. +0.2 -0.1 -0.5
ypd buoyancy 40 hrs Median Buoyancy -0.0 N.D. +0.0 +0.1 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. -0.5 -0.1 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. -1.0 -0.2 +1.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.6 N.D. +0.3 -0.1 -0.4
ypd buoyancy 40 hrs High Buoyancy +0.4 N.D. +0.1 -0.1 +0.8
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