Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9664 and 9665 are separated by 521 nucleotides9665 and 9666 are separated by 302 nucleotides9666 and 9667 are separated by 510 nucleotides9667 and 9668 overlap by 17 nucleotides mRNA_1296: 9664 - K19703 FA2H, SCS7 4-hydroxysphinganine ceramide fatty acyl 2-hydroxylase, at 856,418 to 857,945 9664 mRNA_1297: 9665 - K17292 TBCA tubulin-specific chaperone A, at 858,467 to 858,877 9665 mRNA_1298: 9666 - HMMPfam-Ubiquitin-2 like Rad60 SUMO-like-PF13881,ProSiteProfiles-Ubiquitin domain profile.-PS50053,SUPERFAMILY--SSF54236, at 859,180 to 859,998 9666 mRNA_1299: 9667 - K00600 glyA, SHMT glycine hydroxymethyltransferase, at 860,509 to 862,713 9667 mRNA_1300: 9668 - BLAST polynucleotide 5-hydroxyl-kinase grc3 [Xanthophyllomy..., at 862,697 to 864,122 9668
Group Condition mRNA_1296 mRNA_1297 mRNA_1298 mRNA_1299 mRNA_1300
9664 9665 9666 9667 9668
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -1.3 -0.2 -5.6 -1.1
auxotrophy YNB Glucose 75mM Arginine N.D. -0.5 -0.2 -5.7 -0.4
facs on lipid accumulation Sorted High BODIPY Gate N.D. -1.1 -0.2 -4.5 -1.0
auxotrophy YNB Glucose N.D. -0.4 -0.0 -5.8 -0.3
auxotrophy YNB Glucose 75mM Methionine N.D. -0.5 -0.1 -4.8 -0.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.3 +0.1 -4.6 -0.9
growth on ricinoleic acid YNB Glucose N.D. -0.3 -0.1 -3.8 -0.7
auxotrophy Dropout Complete N.D. +0.1 -0.2 -3.7 -0.6
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.6 -0.2 -3.1 -0.3
growth on oleic acid YNB Glucose N.D. +0.1 -0.1 -3.8 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.0 -0.1 -2.5 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.2 -0.0 -2.2 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.5 -0.2 -2.0 -0.6
growth on ypd YPD N.D. +1.3 -0.2 -2.5 -0.5
growth on oleic acid YNB Oleic Acid N.D. -0.2 -0.1 -0.9 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.3 -0.1 -0.4 -0.6
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.2 -0.1 -0.5 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.5 -0.1 +0.3 -0.4
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 +0.1 -0.3 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -1.0 -0.2 +1.0 +0.0
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 -0.1 +0.8 -0.3
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