Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9665 and 9666 are separated by 302 nucleotides9666 and 9667 are separated by 510 nucleotides9667 and 9668 overlap by 17 nucleotides9668 and 9669 are separated by 805 nucleotides mRNA_1297: 9665 - K17292 TBCA tubulin-specific chaperone A, at 858,467 to 858,877 9665 mRNA_1298: 9666 - HMMPfam-Ubiquitin-2 like Rad60 SUMO-like-PF13881,ProSiteProfiles-Ubiquitin domain profile.-PS50053,SUPERFAMILY--SSF54236, at 859,180 to 859,998 9666 mRNA_1299: 9667 - K00600 glyA, SHMT glycine hydroxymethyltransferase, at 860,509 to 862,713 9667 mRNA_1300: 9668 - BLAST polynucleotide 5-hydroxyl-kinase grc3 [Xanthophyllomy..., at 862,697 to 864,122 9668 mRNA_1301: 9669 - K06947 GRC3, NOL9 polynucleotide 5'-hydroxyl-kinase GRC3/NOL9, at 864,928 to 866,257 9669
Group Condition mRNA_1297 mRNA_1298 mRNA_1299 mRNA_1300 mRNA_1301
9665 9666 9667 9668 9669
facs on lipid accumulation Sorted Low BODIPY Gate -1.3 -0.2 -5.6 -1.1 -0.4
auxotrophy YNB Glucose 75mM Arginine -0.5 -0.2 -5.7 -0.4 +0.4
facs on lipid accumulation Sorted High BODIPY Gate -1.1 -0.2 -4.5 -1.0 +0.6
auxotrophy YNB Glucose -0.4 -0.0 -5.8 -0.3 +0.6
facs on lipid accumulation Sorted No BODIPY Gate -0.3 +0.1 -4.6 -0.9 +0.2
auxotrophy YNB Glucose 75mM Methionine -0.5 -0.1 -4.8 -0.3 +0.6
auxotrophy Dropout Complete +0.1 -0.2 -3.7 -0.6 -0.0
growth on ricinoleic acid YNB Glucose -0.3 -0.1 -3.8 -0.7 +0.6
growth on oleic acid YNB Glucose +0.1 -0.1 -3.8 -0.0 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.6 -0.2 -3.1 -0.3 +1.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 -0.1 -2.5 -0.4 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 -0.0 -2.2 -0.3 +0.6
ypd buoyancy 40 hrs No Buoyancy Separation -1.0 -0.2 +1.0 +0.0 -1.5
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 -0.2 -2.0 -0.6 +0.7
growth on ypd YPD +1.3 -0.2 -2.5 -0.5 +0.3
growth on oleic acid YNB Oleic Acid -0.2 -0.1 -0.9 -0.2 +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.0 +0.1 -0.3 -0.2 +0.2
ypd buoyancy 40 hrs Low Buoyancy +0.2 -0.1 -0.5 -0.3 +0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.5 -0.1 +0.3 -0.4 +0.7
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 -0.1 -0.4 -0.6 +0.8
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.1 +0.8 -0.3 +0.2
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