Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9680 and 9681 are separated by 393 nucleotides9681 and 9682 are separated by 76 nucleotides9682 and 9683 are separated by 310 nucleotides9683 and 9684 are separated by 103 nucleotides mRNA_1312: 9680 - KOG4690 Uncharacterized conserved protein, at 898,611 to 899,248 9680 mRNA_1313: 9681 - K18586 COQ4 ubiquinone biosynthesis protein COQ4, at 899,642 to 900,633 9681 mRNA_1314: 9682 - BLAST carboxymethylenebutenolidase [Rhizoctonia solani AG-1..., at 900,710 to 903,135 9682 mRNA_1315: 9683 - K12813 DHX16 pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16, at 903,446 to 907,323 9683 mRNA_1316: 9684 - KOG4711 Predicted membrane protein, at 907,427 to 911,419 9684
Group Condition mRNA_1312 mRNA_1313 mRNA_1314 mRNA_1315 mRNA_1316
9680 9681 9682 9683 9684
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 N.D. -0.9 N.D. -0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.8 N.D. +0.2 N.D. -0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 N.D. +1.7 N.D. -2.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.9 N.D. +0.1 N.D. +0.3
ypd buoyancy 40 hrs Low Buoyancy -0.3 N.D. -0.2 N.D. -0.0
auxotrophy YNB Glucose 75mM Methionine -0.5 N.D. +0.1 N.D. -0.2
growth on ypd YPD +0.2 N.D. -0.6 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 N.D. -0.6 N.D. +0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.2 N.D. -0.7 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. +0.1 N.D. -0.0
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. +0.3 N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine -0.2 N.D. +0.2 N.D. -0.1
growth on oleic acid YNB Glucose +0.1 N.D. +0.3 N.D. +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. +0.5 N.D. +0.2
growth on ricinoleic acid YNB Glucose -0.5 N.D. +1.2 N.D. -0.1
auxotrophy YNB Glucose +0.2 N.D. +0.6 N.D. +0.0
auxotrophy Dropout Complete +0.3 N.D. +0.4 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. +0.7 N.D. +0.2
growth on oleic acid YNB Oleic Acid +0.1 N.D. +1.1 N.D. +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 N.D. +1.1 N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.5 N.D. +1.8 N.D. +0.1
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