Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9697 and 9698 are separated by 273 nucleotides9698 and 9699 are separated by 362 nucleotides9699 and 9700 are separated by 562 nucleotides9700 and 9701 are separated by 2171 nucleotides mRNA_1329: 9697 - HMMPfam-SUZ domain-PF12752,ProSiteProfiles-SUZ domain profile.-PS51673, at 8,055 to 8,960 9697 mRNA_1330: 9698 - HMMPfam-LCCL domain-PF03815,ProSiteProfiles-LCCL domain profile.-PS50820,SUPERFAMILY--SSF69848, at 9,234 to 11,606 9698 mRNA_1331: 9699 - KOG2273 Membrane coat complex Retromer, subunit VPS5/SNX1, Sorting nexins, and related PX domain-containing proteins, at 11,969 to 15,219 9699 mRNA_1332: 9700 - K00232 E1.3.3.6, ACOX1, ACOX3 acyl-CoA oxidase, at 15,782 to 19,108 9700 mRNA_1333: 9701 - K08286 E2.7.11.- protein-serine/threonine kinase, at 21,280 to 24,099 9701
Group Condition mRNA_1329 mRNA_1330 mRNA_1331 mRNA_1332 mRNA_1333
9697 9698 9699 9700 9701
growth on oleic acid YNB Oleic Acid +0.1 -0.1 N.D. -0.7 N.D.
growth on ypd YPD -0.1 -0.2 N.D. -0.2 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -0.2 N.D. -0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.2 -0.1 N.D. -0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.1 +0.1 N.D. -0.3 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.1 N.D. -0.4 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 -0.1 N.D. -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.0 N.D. -0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.1 N.D. -0.2 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 -0.1 N.D. -0.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 -0.1 N.D. -0.2 N.D.
auxotrophy Dropout Complete +0.0 -0.1 N.D. -0.1 N.D.
growth on oleic acid YNB Glucose +0.1 -0.0 N.D. -0.2 N.D.
auxotrophy YNB Glucose +0.1 +0.2 N.D. -0.2 N.D.
growth on ricinoleic acid YNB Glucose +0.2 +0.2 N.D. -0.4 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 +0.4 N.D. -0.0 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.2 +0.1 N.D. +0.3 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 +0.4 N.D. -0.5 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.3 +0.4 N.D. +0.4 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.3 +0.3 N.D. -0.1 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.6 +0.2 N.D. -0.2 N.D.
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