Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9723 and 9724 are separated by 1196 nucleotides9724 and 9725 are separated by 1229 nucleotides9725 and 9726 are separated by 198 nucleotides9726 and 9727 are separated by 1009 nucleotides mRNA_1355: 9723 - SUPERFAMILY--SSF50685, at 72,198 to 73,021 9723 mRNA_1356: 9724 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 74,218 to 78,285 9724 mRNA_1357: 9725 - BLAST ATP-citrate synthase [Microbotryum lychnidis-dioicae ..., at 79,515 to 80,146 9725 mRNA_1358: 9726 - K01648 ACLY ATP citrate (pro-S)-lyase, at 80,345 to 84,761 9726 mRNA_1359: 9727 - K06018 PPN1 endopolyphosphatase, at 85,771 to 88,302 9727
Group Condition mRNA_1355 mRNA_1356 mRNA_1357 mRNA_1358 mRNA_1359
9723 9724 9725 9726 9727
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 -0.1 -0.3 N.D. -0.9
facs on lipid accumulation Sorted No BODIPY Gate +0.1 -0.1 -0.3 N.D. -1.3
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -0.2 +0.2 N.D. -1.4
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.0 -0.3 N.D. -0.6
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 +0.3 -0.6 N.D. -0.5
auxotrophy YNB Glucose 75mM Methionine -0.0 +0.1 +0.0 N.D. -0.9
auxotrophy Dropout Complete +0.0 +0.2 -0.2 N.D. -0.9
auxotrophy YNB Glucose +0.1 +0.1 -0.2 N.D. -0.5
auxotrophy YNB Glucose 75mM Arginine +0.0 +0.2 -0.1 N.D. -0.5
growth on ypd YPD -0.0 -0.3 +0.1 N.D. -0.2
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.1 +0.1 N.D. -0.4
growth on ricinoleic acid YNB Glucose +0.2 +0.1 -0.1 N.D. -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 +0.3 +0.2 N.D. -1.0
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.1 +0.1 N.D. -0.4
growth on oleic acid YNB Oleic Acid +0.1 +0.0 +0.4 N.D. -0.2
growth on oleic acid YNB Glucose +0.4 -0.0 +0.4 N.D. -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 +0.1 +0.3 N.D. -0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 -0.1 +0.4 N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 +0.3 +0.3 N.D. +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 +0.2 -0.5 N.D. +1.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.6 +0.4 +0.4 N.D. +0.5
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